浸润性乳腺癌ER、PR、HER-2、E-cadherin、CK5/6检测与临床预后关系  

Expression of ER,PR,HER-2,E-cadherin,CK5/6 in the invasive breast cancer and relationship with clinical prognosis

作　　者：谭敏华[1] 雷伟华[1] 谭冬玲[1] 胡志雄[1] 陈威[1] 邹绮嫦[1] 

机构地区：[1]广东省肇庆市第一人民医院病理科,526021

出　　处：《临床与实验病理学杂志》2011年第9期933-938,共6页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨ER、PR、HER-2、E-cadherin、CK5/6检测与浸润性乳腺癌(invasive breast cancer,IBC)临床预后关系。方法应用ER、PR、HER-2、E-cadherin、CK5/6抗体,对64例IBC患者进行免疫组化检测,检测结果后行统计学分析。结果 64例IBC中ER、PR、HER-2、E-cadherin、CK5/6阳性表达率分别为60.93%、56.25%、37.50%、89.06%、10.94%。经统计学分析,ER与PR表达呈正相关(r=0.646,P=0.000);ER与HER-2、CK5/6表达呈负相关(r=-0.320,P=0.010;r=-0.385,P=0.002);PR与HER-2、CK5/6表达呈负相关(r=-0.392,P=0.013;r=-0.362,P=0.003)。采用χ2检验或Fisher精确概率法分析,ER、CK5/6在组织学分级中有统计学意义(P<0.05)。患者在不同年龄、是否绝经、肿瘤大小、组织学分级、有无淋巴结转移及肿瘤分期之间,PR、HER-2和E-cadherin均无统计学意义(P>0.05)。ER、CK5/6在患者的不同年龄、是否绝经、肿瘤大小、有无淋巴结转移及肿瘤分期之间的差异均无统计学意义(P>0.05)。采用Kaplan-Meier法,生存曲线间的比较采用Log-rank检验,总生存率(OS):HER-2或CK5/6阳性患者较阴性患者低(OS:P=0.048和P=0.041)。E-cadherin阳性患者较阴性患者总生存率(OS)高(OS:P=0.038)。但ER、PR的表达与总生存率相关性无统计学意义(OS:P>0.05)。患者的组织学分级越高,总生存率(OS)越低(OS:P=0.048)。患者在不同年龄、是否绝经、肿瘤大小、有无淋巴结转移、临床分期与总生存率(OS)相关性无统计学意义(OS:P>0.05)。结论 IBC中ER、PR、E-cadherin表达丧失,HER-2或CK5/6呈阳性表达,组织学分级呈高级别,提示IBC患者预后较差。联合检测IBC组织中ER、PR、HER-2、E-cadherin、CK5/6的表达,可为IBC临床预后评估提供有力依据。Purpose To investigate the expression of ER,PR,HER-2,E-cadherin,CK5/6 in invasive breast cancer and to study the relationship between their expression with prognosis.Methods Application of ER,PR,HER-2,E-cadherin,CK5/6 antibody in 64 cases of invasive breast cancer patients were performed by immunohistochemistry analysis,results were statistically analyzed.Results The posivite rates of ER,PR,HER-2,E-cadherin,CK5/6 expression in 64 cases of invasive breast cancer were 60.93%,56.25%,37.50%,89.06%,and 10.94% respectively.The statistical analysis showed ER and PR was positive correlation（r= 0.646,P=0.000）.ER and HER-2,CK5/6 expression was negative correlation（r=-0.320,P=0.010;r=-0.385,P=0.002）.PR and HER-2,CK5/6 expression was a negative correlation（r=-0.392,P=0.013;r=-0.362,P=0.003）.Using χ2 test or Fisher exact probability analysis,ER,CK5/6 expression were related to the histological grading（P0.05）.However,PR,HER-2,and E-cadherin expression were unrelated to the age,menopause status,tumor size,histological grade,lymph node metastasis and tumor stage（P0.05）.There was no significant difference between ER,CK5/6 expression and the age,menopause status,tumor size,lymph node metastasis and tumor stage（P0.05）.Log-rank survival curves showed the overall survival（OS） in HER-2 or CK5/6 positive patients was lower than that in HER-2 or CK5/6 negative patients（OS： P= 0.048 and P=0.041）.The OS in E-cadherin positive patients was higher than that E-cadherin negative patients（OS： P= 0.038）.But the correlation between ER,PR expression with overall survival was no significant difference（OS： P0.05）.OS in the patients with higher histological grade was lower（OS： P=0.048）.The age,menopause status,tumor size,lymph node metastasis,clinical stage and overall survival（OS） had no significant correlation（OS： P0.05）.Conclusion ER,PR,E-cadherin expression loss,HER-2 or CK5/6 positive expression,higher histological grade suggest poor prognosis of invasive breast cancer.The combined

关 键 词：乳腺浸润性肿瘤 免疫组化 临床病理特征 预后关系 

分 类 号：R737.9[医药卫生—肿瘤]