淋巴瘤组织和血浆中TNT-α表达及其临床意义  

Clinical significance of TNF-α expression in lymphoma tissue and plasma

作　　者：马瑞[1] 哈德提.别克米托夫 顾霞[1] 陈海霞[1] 王欣[1] 

机构地区：[1]新疆医科大学第一附属医院病理科,乌鲁木齐830054

出　　处：《临床与实验病理学杂志》2011年第9期939-943,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(30860096)

摘　　要：目的探讨不同类型淋巴瘤患者组织和血浆肿瘤坏死因子α(TNF-α)表达状况,以及与临床预后参数的关系。方法收集97例淋巴瘤患者石蜡组织和血浆标本,用Super Vision两步法免疫组化和ELISA法分别检测TNF-α在组织和血浆中的表达。结果 TNF-α在不同类型的淋巴瘤组织中均有不同程度的表达,组间比较浆细胞肿瘤(plasma cell neoplasm,PCN)与淋巴母细胞性淋巴瘤(lymphoblastic lymphoma,LBL)、LBL与外周T细胞淋巴瘤(peripheral T-cell lymphoma,PTL)组间差异有显著性(P<0.05)。血浆TNF-α水平除PCN和LBL外,淋巴瘤患者组皆高于对照组,其中霍奇金淋巴瘤(Hodgkin’s lymphoma,HL)、小淋巴细胞淋巴瘤(small lymphocytic lymphoma,SLL/CLL)、滤泡性淋巴瘤(follicular lymphoma,FL)、NK/T细胞淋巴瘤(NK/T cell lymphoma,NK/TCL)和套细胞淋巴瘤(mantle cell lymphoma,MCL)与对照组间的差异有显著性(P<0.05)。血浆中TNF-α表达在淋巴瘤患者男性组、Ann Ardor分期Ⅳ、国际预后指数(international prognostic index,IPI)高危组、有B症状组间表达明显高于女性组、Ann Ardor分期Ⅰ～Ⅲ、IPI低、中危、无B症状组,组间差异有显著性(P<0.05)。在不同年龄、民族、乳酸脱氢酶(lactic dehydrogenase,LDH)、C反应蛋白(C-reactive protein,CRP)、β2-微球蛋白(β2-microglobulin,β2-MG)间的差异无显著性(P>0.05);而组织中TNF-α表达与以上临床参数不同组间差异皆无显著性(P>0.05)。淋巴瘤患者TNF-α血浆和组织中的表达无相关关系(r=0.119,P>0.05)。结论 TNF-α在多种类型淋巴瘤患者中组织和血浆中高表达,说明TNF-α可能在淋巴瘤的发生、发展中起着作用。血浆中TNF-α水平的检测对淋巴瘤临床预后判断有一些参考价值。Purpose To investigate expression of tumor necrosis factor α（TNF-α） in lymphoma tissue and plasma,as well as the relationship between clinical outcome and prognosis of lymphoma.Methods 97 cases of lymphoma paraffin tissue and plasma samples were collected,and the expression of TNF-α in tissue and plasma were used by immunohistochemical methods and ELISA.Results Variable expression of TNF-α was observed in various subtypes of lymphoma which there were significant difference between plasma cell neoplasm（PCL） and lymphoblastic lymphoma（LBL）,LBL and peripheral T-cell lymphoma（PTL）（P0.05）.All plasma levels of TNF-α group in lymphoma patients were higher than control group,except for LBL and PCN,which Hodgkin＇s lymphoma（HL）、small lymphocytic lymphoma（SLL）、follicular lymphoma（FL）、NK/T cell lymphoma（NK / TCL）,mantle cell lymphoma（MCL） were higher than that in control group（P0.05）.The expression of TNF-α in male patients、Ann Ardor stage Ⅳ,IPI index of high-risk group and the group of B symptoms were significantly higher than the expression of women、 Ann Ardor stageⅠ-Ⅲ,IPI low-intermediate risk index and no B symptoms group（P0.05）.There were no difference at different ages,ethnic,LDH,CRP,β2-MG（P0.05）.There were not difference between the expression of TNF-α in tissue and clinical parameters of the different groups（P0.05）.The expression of TNF-α in plasma was negatively correlated to that of tissues（r=0.119,P0.05）.Conclusion The high expression of TNF-α in the patients with lymphoma may be correlated to the development of lymphoma.Plasma levels of TNF-α may more meaningful to evaluate clinical prognosis.

关 键 词：淋巴瘤 TNF-Α ELISA 免疫组化 

分 类 号：R733.4[医药卫生—肿瘤]