羊源巴贝斯虫未定种甘肃敦煌株的发现  

Discovery of an ovine Babesia sp. strain from sheep in Dunhuang City of Gansu Province

作　　者：马米玲[1] 关贵全[1] 苟惠天[1] 杨吉飞[1] 万玉林[1] 李有全[1] 刘爱红[1] 任巧云[1] 陈泽[1] 王朝文[1] 刘志杰[1] 罗建勋[1] 殷宏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部草食动物疫病重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2011年第9期881-885,共5页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(30800820;30972182;31072130;31001061);甘肃省重点项目(1002NKDA035;0801NKDA033);欧盟项目EPIZONE(FOOD-CT-2006-016236);ASFRISK(211691);ARBOZOONET(211757)和PIROVAC(KBBE-3-245145)

摘　　要：采用动物接种法将采自甘肃敦煌的15只绵羊血液混合后感染1只除脾绵羊,感染后逐日做血涂片检查。结果显示,在感染后第10天的血片上出现了1种大型巴贝斯虫。虫体形态以双梨子形、单梨子形、圆形及卵圆形为主。在感染后第12天,感染羊体温升至41.1℃。染虫率达到0.125%,之后逐渐下降。无菌采集该羊血液,提取全血基因组,用梨形虫通用引物进行PCR扩增全长18SrRNA基因,得到长约1 700bp的片段,将其克隆并测序(登录号:HQ730762),与GenBank中的其他巴贝斯虫的序列进行比较,发现其与新疆巴贝斯虫未定种的同源性最高,达到99.8%。本研究发现甘肃敦煌又是一个新疆羊巴贝斯虫未定种的疫源地。Mixture of field-collected blood from 15 sheep in Dunhuang City was injected into a spleenectomized sheep.A large number of Babesia were present in the blood smeared on day 10 post-inoculation.The major morphological forms included paired piriform,single piriform,ring form and oval form.On day 12 post-inoculation,the body temperature and parasitemia of sheep reached 41.1 ℃ and 0.125% respectively and then decending.Genomic DNA was extracted from whole blood sterilely collected from the infected sheep.A 1 700 bp fragment was amplified by PCR using universal primers based on the 18 S rRNA gene.Then the target fragment was cloned and sequenced（GenBank accession number：HQ730762）.Multiple sequence alignments were performed with those of other Babesia in GenBank.It shared the highest similarity with Babesia sp.Xinjiang,which was up to 99.8%.The present study discovered another epidemic focus of Babesia sp.Xinjiang in Dunhuang City of Gansu Province.

关 键 词：绵羊血液 巴贝斯虫 染虫率 18S RRNA 聚合酶链反应 

分 类 号：S852.723[农业科学—基础兽医学]