天祝白牦牛MSTN基因第3外显子的多态性分析  

Polymorphism analysis of the exon 3 of MSTN gene in Tianzhu White yak

作　　者：闫永红[1,2] 刘霞[1,2] 刘英[3] 罗玉柱[4,2] 杜晓华[3,2] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃省草食动物生物技术重点实验室,甘肃兰州730070 [3]甘肃农业大学动物医学院,甘肃兰州730070 [4]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《中国兽医科学》2011年第9期945-949,共5页Chinese Veterinary Science

基　　金：国家科技支撑计划项目(2009BAC53B06);甘肃省重点实验室建设计划项目(085RTSA004);甘肃省教育厅科研项目(1002B-01)

摘　　要：为分析天祝白牦牛MSTN基因第3外显子的遗传多态性及变异特征,采用PCR-SSCP方法检测了698头天祝白牦牛MSTN基因第3外显子的多态性,对群体内各等位基因进行了克隆、测序。结果显示,天祝白牦牛MSTN基因第3外显子有AA、AB和BB 3种基因型,其基因型频率分别为0.892 6、0.106 0和0.001 4,由A和B 2个等位基因控制。序列分析表明,天祝白牦牛MSTN基因第3外显子在41bp处发生了A→G突变,但并未导致编码氨基酸序列发生改变。统计结果表明,天祝白牦牛MSTN基因第3外显子呈低度多态。In order to study the polymorphism and variation of exon 3 of MSTN gene in Tianzhu White yak,DNA fragments containing MSTN gene exon 3 were amplified and analysed using PCR single-strand conformational polymorphism（SSCP）,DNA sequence analysis were used to detect genetic variation.The results showed that the polymorphism of the exon 3 of MSTN gene in Tianzhu White yak was controlled by A and B alleles which formed three genotypes namely AA,AB and BB,and the frequencies of them were 0.892 6,0.106 0 and 0.001 4,respectively.Sequence analysis indicated that there was one single nucleotide mutation A to G at 41 bp,which was a synonymous mutation.Statistical results showed that MSTN gene exon 3 in Tianzhu White yak has low level of sequence polymorphism.

关 键 词：MSTN基因 遗传多态性 聚合酶链反应-单链构象多态性 白牦牛 

分 类 号：S823.85[农业科学—畜牧学]