Glutamine analogs promote cytoophidium assembly in human and Drosophila cells  

作　　者：mr Yilmaz Tastan Zillah Anne Deussen Mayte Yu-Yin Siswick 

机构地区：[1]Medical Research Council Functional Genomics Unit,Department of Physiology Anatomy and Genetics,University of Oxford,Oxford OXI 3QX,United Kingdom

出　　处：《Journal of Genetics and Genomics》2011年第9期391-402,共12页遗传学报（英文版）

基　　金：the TRiP at Harvard Medical School(NIH/ NIGMS R01-GM084947)

摘　　要：CTP synthase is compartmentalized within a subcellular structure,termed the cytoophidium,in a range of organisms including bacteria, yeast,fruit fly and rat.Here we show that CTP synthase is also compartmentalized into cytoophidia in human cells.Surprisingly,the occurrence of cytoophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-L-norleucine（DON）,an inhibitor of glutamine-dependent enzymes including CTP synthase.Experiments in flies confirmed that DON globally promotes cytoophidium assembly.Clonal analysis via CTP synthase RNA interference in somatic cells indicates that CTP synthase expression level is critical for the formation of cytoophidia.Moreover,DON facilitates cytoophidium assembly even when CTP synthase level is low.A second glutamine analog azaserine also promotes cytoophidum formation.Our data demonstrate that glutamine analogs serve as useful tools in the study of cytoophidia.CTP synthase is compartmentalized within a subcellular structure,termed the cytoophidium,in a range of organisms including bacteria, yeast,fruit fly and rat.Here we show that CTP synthase is also compartmentalized into cytoophidia in human cells.Surprisingly,the occurrence of cytoophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-L-norleucine（DON）,an inhibitor of glutamine-dependent enzymes including CTP synthase.Experiments in flies confirmed that DON globally promotes cytoophidium assembly.Clonal analysis via CTP synthase RNA interference in somatic cells indicates that CTP synthase expression level is critical for the formation of cytoophidia.Moreover,DON facilitates cytoophidium assembly even when CTP synthase level is low.A second glutamine analog azaserine also promotes cytoophidum formation.Our data demonstrate that glutamine analogs serve as useful tools in the study of cytoophidia.

关 键 词：AZASERINE CTP synthase Cytoophidium 6-diazo-5-oxo-L-norleucine DROSOPHILA HeLa cell lntracellular compartmentation 

分 类 号：Q26[生物学—细胞生物学]