胃癌多药耐药相关基因表达与胶滴肿瘤药物敏感的关系  

Correlation between the expression of multidrug resistance-related genes and collagen gel droplet culture drug sensitivity in gastric cancer

作　　者：韩旭[1] 谭志军[2] 姜伟[2] 

机构地区：[1]天津医科大学第一中心临床学院,300192 [2]天津市第一中心医院普通外科,300192

出　　处：《新医学》2011年第9期568-570,583,F0003,共5页Journal of New Medicine

基　　金：天津市卫生局科技基金(07KY30)

摘　　要：目的:探讨胃癌组织中多药耐药相关基因产物P-糖蛋白(P-gp)、谷胱甘肽-S-转移酶π(GST-π)和胸苷酸合成酶(TS)的表达与胶滴肿瘤药物敏感性之间的关系。方法:收集53例手术切除胃癌患者的胃癌标本,应用胶滴肿瘤药物敏感检测技术(CD-DST)检测其对4种临床常用化学治疗药物(多柔比星、依托泊苷、氟尿嘧啶、奥沙利铂)的体外药物敏感性,并应用免疫组化法检测胃癌组织中P-gp、GST-π和TS的表达,分析其与药敏检测结果的关系。结果:P-gp、GST-π和TS在胃癌组织中的阳性表达率分别为58%、47%和40%。P-gp阳性表达者中多柔比星及依托泊苷耐药组所占比例明显高于敏感组(P<0.05);GST-π阳性表达者中多柔比星及奥沙利铂耐药组所占比例也明显高于敏感组(P<0.05);TS阳性表达者中氟尿嘧啶耐药组和敏感组所占比例差异有统计学意义(P<0.05)。结论:P-gp、GST-π和TS高表达是胃癌对多种化学治疗药物具有耐药性的标志,结合体外药物敏感试验,将有助于临床筛选有效的化学治疗药物,实现胃癌的个体化化学治疗。Objective：To investigate the correlation between the expression of multidrug resistance-related genes,including P-glycoprotein（P-gp）,glutathione-S-transferase π（GST-π） and thymidylate synthase（TS）,and collagen gel droplet culture drug sensitivity in gastric carcinoma tissues.Methods：Gastric carcinomas tissues were surgically obtained from 53 patients.The chemosensitivity to four anticancer drugs（doxorubicin,etoposide,fluorouracil and oxaliplatin） was assessed by collagen gel droplet culture drug-sensitivity test（CD-DST）;while the expression of P-gp,GST-π and TS in gastric carcinoma tissues was assessed by immunohistochemistry for correlation analysis.Results：The positive expression rates of P-gp,GST-π and TS were 58%,47% and 40%,respectively.Significantly higher doxorubicin and etoposide-resistance rates were revealed in P-gp positive group than those in P-gp negative group（P〈0.05）;so were the doxorubicin and oxaliplatin-resistance in GST-π positive group（P〈0.05）;so was the fluorouracil-resistance rate TS group（P〈0.05）.Conclusion：Overexpression of P-gp,GST-π and TS is involved in multidrug resistance of gastric cancer,thus providing auxiliary technique for chemosensitivity in vitro in chemotherapeutic drug selection for gastric cancer.

关 键 词：胶滴肿瘤药物敏感检测技术 胃癌 多药耐药 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]