Stat3、Bcl-2及cyclinD1在口腔黏膜癌前病变癌变中表达的相关性研究  

Expression of Stat3,Bcl-2 and cyclinD1 in process of malignant change of oral premalignant lesions

作　　者：李留炀[1,2] 吴国英[1,2] 杨筱荣[1,2] 汤根兄[3] 杨静[1,2] 

机构地区：[1]南京医科大学口腔医学研究所 [2]南京医科大学附属口腔医院牙髓黏膜科,江苏南京210029 [3]南京医科大学附属南京市儿童医院口腔科,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2011年第9期1360-1363,1368,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅自然科学研究基金(03KJD320148)

摘　　要：目的:研究口腔黏膜癌前病变癌变过程中Stat3、Bcl-2及cyclinD1的表达及其相关性。方法:采用免疫组化的方法分别检测正常口腔黏膜、单纯性增生黏膜、异常增生黏膜与口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中Stat3、Bcl-2及cyclinD1的表达。结果:正常口腔黏膜、单纯性增生黏膜、异常增生黏膜和OSCC中Stat3和Bcl-2、cyclinD1的积分光密度值(intergrated optical density,IOD)均逐步升高;Stat3、Bcl-2、cyclinD1三者的IOD值除单纯性增生黏膜与口腔正常黏膜无统计学意义外,其余各组间均有统计学意义。Stat3与Bcl-2 IOD值呈线形正相关;Stat3与cyclinD1 IOD值呈线形正相关;Bcl-2与cy-clinD1 IOD值无相关性。结论:Stat3、Bcl-2及cyclinD1在口腔黏膜癌前病变癌变中表达的相关性提示,口腔黏膜癌前病变癌变是由多种基因共同参与的过程。Objective：To study the expression of Stat3,Bcl-2 and cyclinD1 in process of malignant change of oral premalignant lesions.Methods： Expression of Stat3 and cyclinD1 were detected in normal oral epithelia,simple hyperplasia epithelia,dysplasia epithelia and oral squamous cell carcinoma（OSCC） by immunohistochemical assay.Results： From normal mucosa,hyperplasia epithelia,dysplasia epithelia to OSCC,the value of IOD increased stepwise.There were statistical significances of Stat3 expression between every two groups in all groups except for between normal mucosa and hyperplasia epithelia groups,as well as expressions of Bcl-2 and cyclinD1.The expression of Stat3 was linear positively correlated to that of Bcl-2 and cyclinD1.No relationship was found between Bcl-2 and cyclinD1.Conclusion： The relationships among Stat3,Bcl-2 and cyclinD1 indicate that more than one genes participate in the process of malignant change of oral premalignant lesions.

关 键 词：STAT3 BCL-2 CYCLIND1 癌前病变 口腔 鳞状细胞癌 

分 类 号：R739.85[医药卫生—肿瘤]