恶臭假单胞菌(Pseudomonas putida LY1)共代谢降解苯酚和4-氯苯酚系统的降解动力学研究  

Kinetic study on cometabolic biodegradation of phenol and 4-chlorophenol by Pseudomonas putida LY1

作　　者：李晶[1,2] 饶婷[1] 李巍[1] 李轶[1] 

机构地区：[1]河海大学环境学院浅水湖泊综合治理与资源开发教育部重点实验室,南京210098 [2]泰州市环境科学研究所,泰州225300

出　　处：《环境科学学报》2011年第10期2109-2116,共8页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金项目(No.51009050)~~

摘　　要：对恶臭假单胞菌(Pseudomonas putida LY1)共代谢降解苯酚和4-氯苯酚(4-CP)系统进行了降解实验和动力学研究.结果表明,恶臭假单胞菌可以有效地降解苯酚,苯酚浓度为50mg·L-1时细菌生长速度最快,4-氯苯酚浓度的增加会对细菌产生一定的抑制作用.同时,用改进的Haldane方程模拟恶臭假单胞菌LY1单独降解苯酚的过程,并根据实验数据利用MATLAB软件求出了方程参数值为:最大比降解速率Rm=0.113mg·mg-·1h-1,饱和常数Ks=3.5mg·L-1,抑制常数Kp=126.6mg·L-1.在此基础上修正改进的Haldane方程用以模拟恶臭假单胞菌共代谢降解苯酚和4-氯苯酚,利用MATLAB软件并结合6组共代谢降解实验(PC1～PC2,PC5～PC8)数据解出未知参数K1,n1,K2,n2,发现参数改进的Haldane方程经过修正后能很好地模拟恶臭假单胞菌共代谢降解苯酚和4-氯苯酚的过程.该模型为污水中难降解有机物的生物降解提供了理论参考.Degradation of phenol and 4-chlorophenol by Pseudomonas putida LY1 was studied.It was found that phenol can be effectively degraded by the bacteria with an optimum phenol concentration at 50 mg.L-1.Inhibition of the bacteria increased with the 4-chlorophenol concentration.A modified Haldane equation was used to study the biodegradation of phenol by Pseudomonas putida LY1.The equation parameters calculated using MATLAB software were as follows：Rm = 0.113 mg.mg-1.h-1,Ks = 3.5 mg.L-1 and Kp = 126.6 mg.L-1.The modified Haldane equation was also used to simulate the cometabolic biodegradation of phenol and 4-chlorophenol by Pseudomonas putida LY1.The cometabolic degradation model can simulate the process of cometabolic degradation very well.The model provides a theoretical and technical reference for the biodegradation of chlorinated refractory organic pollutants in wastewater treatment plants.

关 键 词：共代谢降解 4-氯苯酚 苯酚 动力学 恶臭假单胞菌 

分 类 号：X172[环境科学与工程—环境科学]