靶向phTERT-PE38KDEL重组质粒联合硼替佐米抑制裸鼠胰腺癌移植瘤生长的研究  

Effects of recombinant plasmid phTERT-PE38KDEL combined bortezomib on inhibiting the transplanted pancreatic tumor growth in athymic mice

作　　者：邢帅[1] 孙晋津[1] 李宝玉[1] 孙宁[1] 陈剑秋[1] 

机构地区：[1]天津医科大学第二医院,300211

出　　处：《实用医学杂志》2011年第19期3495-3497,共3页The Journal of Practical Medicine

摘　　要：目的:探讨重组质粒phTERT-PE38KDEL联合硼替佐米对裸鼠胰腺癌移植瘤生长的作用。方法:采用人胰腺癌MiaPaCa2细胞系建立荷瘤裸鼠模型,通过腹腔给药和瘤内注射方式,以硼替佐米联合phTERT-PE38KDEL对荷瘤鼠进行治疗,比较各组肿瘤体积和质量,计算抑瘤率。免疫组化法检测核增殖抗原及微血管密度,TUNEL染色检测肿瘤的凋亡。结果:联合治疗组(A组)的肿瘤体积及瘤重在每个检测点均小于基因治疗组(B组)、单纯化疗组(C组)和空白对照组(D组)(P<0.01),与D组相比,A、B、C组肿瘤抑制率分别为:68.98%、51.44%、16.39%。与B和C组相比较,A组的瘤体PCNA增殖指数与MVD显著降低(P<0.01),A组的细胞凋亡数显著增多(P<0.01)。结论:phTERT-PE38KDEL与硼替佐米对裸鼠胰腺癌移植瘤均有抑制作用,二者联用效果更佳。Objective To explore the effects of recombinant plasmid phTERT-PE38KDEL and bortezomib on the growth of transplanted pancreatic tumor in athymic mice.Methods Human pancreatic carcinoma cell line MiaPaCa2 was planted into nude mice to establish the xenografts tumor model By intraperitoneal administration and intratumoral injection,the xenografts tumor mice were treated with bortezomib combined phTERT-PE38KDEL.The tumor volume and weight in all groups were measured,and the tumor inhibitory rate was calculated.The proliferating cell nuclear antigen and microvascular density were examined by immunohistochemistry.Cell apoptosis was observed by TUNEL assay.Results The tumor volume and weight of the combined therapeutic group （Group A） was significantly smaller than those of the gene therapeutic group （Group B）,chemotherapy group（Group C） and control group （Group D） at each time point （P 0.01）.Compared with Group D,the inhibitory rate of tumor growth in Group A,B and C were 68.98%,51.44% and 16.39%,respectively.The PCNA proliferation index and MVD of Group A were significantly lower than those of Group B and C （P 0.01）.Compared with Group B and C,the apoptosis cells in Group A were significantly increased（P 0.01）.Conclusion Both phTERT-PE38KDEL and bortezomib have antitumor effects in vivo and the combined therapy has a stronger antitumor effect.

关 键 词：胰腺肿瘤 HTERT启动子 PE38KDEL基因 硼替佐米 

分 类 号：R735.9[医药卫生—肿瘤]