东北玉簪多糖提取分离及组成研究  

Study on the Isolation and Composition of Hosta ensata Polysaccharides

作　　者：柳怀玉[1] 王莲萍[2] 丁云录 李庆杰[3] 杨丽 南敏伦 

机构地区：[1]吉林大学实验动物中心,吉林长春130118 [2]吉林农业大学,吉林长春130118 [3]吉林天药科技有限公司,吉林长春130012 [4]内蒙古自治区人民医院中药房,内蒙古呼和浩特010017 [5]吉林省中医药科学研究院,吉林长春130012

出　　处：《安徽农业科学》2011年第26期15934-15935,15938,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(2007BAI38B05)

摘　　要：[目的]研究玉簪多糖的提取分离及其组成。[方法]利用水提醇沉法得粗多糖,并依次用30%、50%、80%乙醇分部沉淀得多糖CP1、CP2、CP3;经Superdex-100凝胶柱色谱分离得CP4、CP5。采用光谱法和色谱法对其总糖含量、单糖组成等进行了分析。[结果]东北玉簪5个多糖样品酸性糖含量为20.36%~52.76%、中性糖含量为34.07%~55.62%。[结论]东北玉簪多糖CP1~CP5均为中性糖和酸性糖的混合物。[Objective]To study the isolation and composition of polysaccharides from Hosta ensata.[Method]Crude polysaccharide was obtained by water extraction and then precipitated respectively by 30%,50% and 80% alcohol to get CP1,CP2 and CP3,CP4 and CP5 were isolated through Superdex G-100 gel column chromatography.Content of total sugar and composition of monose were analyzed by using spectrum and chromatography.[Result]Of five polysaccharide samples from Hosta ensata,the content of acidic polysaccharides ranged from 20.36% to 52.76%,while that of neutral polysaccharides was in the range of 34.07%-55.62%.[Conclusion]All CP1-CP5 from Hosta ensata were the mixture of acid and neutral sugar.

关 键 词：东北玉簪 多糖 提取分离 

分 类 号：S37[农业科学—农产品加工]