嗜热厌氧杆菌X514在不同氮源培养基中的生长及代谢研究  

Study of Growth and Metabolism of Thermoanaerobacter sp. X514 under Different Nitrogen Sources

作　　者：谢天[1] 

机构地区：[1]中国科学院青岛生物能源与过程研究所,山东青岛266101

出　　处：《安徽农业科学》2011年第26期16061-16062,16103,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30800202)

摘　　要：[目的]研究适宜嗜热厌氧杆菌X514生长和代谢的氮源培养基添加物。[方法]比较X514在添加不同氮源添加物(有机氮源蛋白胨、酵母提取物和无机氮源NH4C l)的基本培养基中的生长和代谢。[结果]酵母提取物和蛋白胨相对于无机氮源都能促进X514的生长,其最大生长速率是0.150 h-(添加5 g/L的酵母提取物培养基),同时X514在高浓度蛋白胨培养基中(5 g/L)的生长相对于低浓度蛋白胨培养基(0.5 g/L)延迟4 h左右;酵母提取物能有效地促进X514产乙醇,与含等量的蛋白胨培养基相比其乙醇转换率(mol产出乙醇/mol消耗的葡萄糖)平均高30%左右。[结论]酵母提取物可以更高效地促进X514的生长和乙醇产出。[Objective] The aim was to find out the appropriate nitrogen medium supplement for Thermoanaerobacter sp.X514.[Method] After comparing the growth and mechanism of X514 in media added with different nitrogen medium supplements（including the organic medium supplements：trptone and yeast extract and inorganic medium supplement NH4Cl）,a suitable medium supplement for X514 was found.[Result]The grwoth results in various media added with 5 g/L glucose showed that trptone and yeast extract could promote the growth of X514 compared to NH4Cl;the maximum growth rate was 0.15 h-（the basal medium added with 5 g/L yeast extract）;and the growth of X514 in the medium with high concentration of trytone（5 g/L） was delayed for 4 hours compared to it in the medium with lower concentration of tryptone（0.5 g/L）.Finally,after analyzing the product distribution in those media,yeast extract was more effective in promting the ethanol production and the ethanol conversation rates（mol ethanol produced/ mol glucose consumed） in media added with yeast extract was 30% more than those in media added with equal amount of tryptone on average.[Conclusion]Through those experiments,it was found that compared with the equal amount of tryptone,yeast extract was more effective in promoting the growth and ethanol production of X514.

关 键 词：嗜热厌氧杆菌 蛋白胨 酵母提取物 

分 类 号：X74[环境科学与工程—环境工程]