三种试剂盒提取粪便隐孢子虫微量DNA的效果比较  

Comparison of Extraction Effects of Cryptosporidium Micro DNA from Excrement by Three Kits

作　　者：赵梅珍[1] 刘晖[1] 贺莉芳[1] 马元芬[1] 

机构地区：[1]遵义医学院,贵州遵义563003

出　　处：《安徽农业科学》2011年第26期16130-16131,共2页Journal of Anhui Agricultural Sciences

基　　金：国家卫生公益行业科研专项(200802012);贵州省科技基金项目[黔科合J字(2008)2194]

摘　　要：[目的]筛选一种高效、简单、快速、价格合理的粪便微量DNA提取试剂盒。[方法]用3种不同的试剂盒提取粪便DNA,通过比较所提取DNA的浓度、纯度、提取率及巢式PCR产物等评价提取效果。[结果]Genmed试剂盒提取的DNA浓度最高、PCR产物条带最亮,但提取时间相对较长,成本相对较高。天根试剂盒提取的DNA纯度最高,DNA浓度适中,成本适中,提取时间最短。碧云天试剂盒提取的DNA纯度、浓度均不高且提取时间最长,但成本最低。[结论]Genmed试剂盒提取粪便微量DNA最灵敏。天根试剂盒提取DNA适于DNA纯度要求较高的分子生物学研究。[Objective] To choose an efficient,simple,fast and affordable excrement micro DNA extraction kit.[Method] Excrement DNA was extracted by three different kits,the extraction effects of which were evaluated through comparing the concentration,purity,extraction rate and nested PCR products of DNA.[Result] The concentration of DNA extracted by Genmed kit was the highest,PCR product brand was the brightest,but the extraction time was too long and the price was high.The purity of DNA extracted by TIANGEN kit was the highest,DNA concentration and price were moderate,and the extraction time was the shortest.Both the purity and concentration of DNA extracted by Beyotime kit were not high and the extraction time was the longest,but the price was the lowest.[Conclusion] Genmed kit is the most sensitive in extracting excrement micro DNA;TIANGEN kit wis applicable to the molecular biological research which calls for higher DNA purity.

关 键 词：粪便DNA DNA提取 试剂盒 

分 类 号：R384.2[医药卫生—医学寄生虫学]