饲料中沙门氏菌的分离鉴定与Dot-ELISA检测  

Detection Salmonella in Forage by Dot-ELISA

作　　者：韩志辉[1] 张红见[1] 

机构地区：[1]青海大学农牧学院动物医学系,青海西宁810016

出　　处：《安徽农业科学》2011年第26期16139-16140,16163,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立检测饲料中沙门氏菌的Dot-ELISA法。[方法]采用Dot-ELISA法,对随机采集自西宁市某饲料厂的100份成品饲料、85份鱼粉和50份精蛋白进行了沙门氏菌检测。通过生化试验和血清学试验对饲料样品中的细菌进行了进一步的鉴定。[结果]通过Dot-ELISA检测,成品饲料、鱼粉和精蛋白中的沙门氏菌阳性率分别为38.00%、62.35%和60.00%。Dot-ELISA法与常规分离鉴定技术的符合率为82.23%。[结论]Dot-ELISA法具有较好的特异性和敏感性,与常规分离技术具有较高的符合率,可以用于饲料中沙门氏菌的检测。[Objective] To develop a Dot-ELISA(Dot-enzyme linked immunosorbent assay) method for detection of Salmonella in forage.[Method] Forage samples including 100 finished feed samples,85 fishmeal samples and 50 protamine samples were randomly collected from a feed factory in Xining City,and Salmonella in these samples were detected by Dot-ELISA.The Salmonella isolates were further identified using biochemical tests and serological tests.[Result] As detected by Dot-ELISA,the positive rates of Salmonella in the finished feed,fishmeal and protamine were 38.00%,62.35% and 60.00%,respectively.The coincidence rate between the Dot-ELISA and routine identification method was 82.23%.[Conclusion] The Dot-ELISA has good specificity and sensitivity and is highly coincidental with the routine identification method.Therefore,Dot-ELISA can be used for detection of Salmonella in forage.

关 键 词：DOT-ELISA 沙门氏菌 检测 饲料 

分 类 号：S831.5[农业科学—畜牧学]