木质纤维素降解酶系的基础和技术研究进展  

Progress in basic and technological research of enzyme system for lignocellulosics biodegradation

作　　者：曲音波[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《山东大学学报（理学版）》2011年第10期160-170,共11页Journal of Shandong University(Natural Science)

摘　　要：山东大学微生物学科从事纤维素降解研究已有50多年的历史。在木质纤维素微生物降解机理研究的基础上,从自然界中筛选到一株能高效降解植物纤维类生物质的斜卧青霉菌株,并获得该菌的多株抗降解物阻遏纤维素酶高产突变菌株。最近,已完成了对多株斜卧青霉菌株的全基因组测序,正在利用系统生物学技术深入探讨其酶系组成和酶合成调控机理。选育出的高产突变株,已用于规模化生产工业用酶,并开发出玉米芯生物炼制生产纤维素乙醇技术。Research on cellulose biodegradation has been carried out for more than 50 years in Shandong University. Based on the mechanism studies on microbial degradation of lignocellulosics, a Penicillium decumbens strain, which can hydrolyze lignocellulose efficiently, was isolated from natural samples, and several catabolite repression-resistant mutants were obtained. Genome sequencing of the strains were carried out, and the composition of their enzyme system and the mechanism for regulation of enzyme synthesis were studied by systems biotechnological methods. The cellulaseoverproducing mutants have been used for cellulase production in industrial scale, and for cellulosic ethanol production in the biorefinery of corncobs.

关 键 词：纤维素酶系 斜卧青霉 纤维素乙醇 生物炼制 

分 类 号：Q55[生物学—生物化学]