维生素A缺乏对大鼠铁调节蛋白2影响  

Effect of vitamin A deficiency on iron regulatory protein 2 in rats

作　　者：姜珊[1] 王朝旭[1] 姜丽英[1] 李丹娜[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,黑龙江哈尔滨150086

出　　处：《中国公共卫生》2011年第10期1271-1272,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30872107);黑龙江省自然科学基金(D200641)

摘　　要：目的探讨维生素A缺乏对大鼠铁调节蛋白2(IRP2)mRNA及铁蛋白(Fn)mRNA和转铁蛋白受体(TfR)mRNA表达影响。方法 48只雄性SD大鼠按体重随机分为4组,每组12只,对照组、维生素A完全缺乏组;维生素A边缘缺乏组;铁及维生素A边缘缺乏组;喂养8周后,麻醉处死大鼠,取组织和血清,进行相关指标和基因表达检测。结果与对照组(1.50±0.41)μmol/L比较,维生素A完全缺乏大鼠血清维生素A(0.22±0.26)μmol/L水平明显降低(P<0.05);与对照组比较,维生素A完全缺乏组大鼠血清铁(2.26±0.72)μg/mL明显降低;维生素A完全缺乏组大鼠肝脏IRP2、TfR表达水平分别为(1.53±0.18)、(0.62±0.06)与对照组相比明显升高,FnmRNA(0.52±0.08),明显低于对照组(P<0.05)。结论维生素A缺乏通过改变IRP2表达、IRP-RNA结合活性,在转录后水平改变TfR和Fn mRNA表达,影响铁稳态。Objective To explore the potential role of iron regulator protein 2（IRP2） under conditions of vitamin A deficiency and the relationship between vitamin A deficiency and anemia.Methods Forty-eight SD rats were divided randomly into four groups of control,complete deficient in vitamin A,marginal deficiency in vitamin A,and ion plus vitamin A marginal deficiency.After 8 weeks,all the rats were killed by sodium pentobarbital anesthesia and all samples were collected and detected for gene expression.Results As expected,the concentration of serum retinol in completely vitamin A deficiency group（0.22±0.26 μmol/L）,compared with that of the control group（1.50±0.41μmol/L）,was significantly decreased.Compared with that of the control group（3.85±0.94μg/ml）,the concentration of serum rion（2.26±0.72 μg/ml） in complete vitaminA deficiency group was significantly decreased.Compared with that of the control group（0.81±0.08）,the level of IRP2 mRNA（1.53±0.18）in complete vitamin A deficiency group was significantly increased,and the level of transferrin receptor mRNA（0.62±0.06） was also increased compared with that of the control group（0.33±0.02）.Compared with the control group（0.85±0.04）,the level of ferrintin RNA（0.52±0.08） in complete vitamin A deficiency group was significantly decreased.Conclusion The results indicate that vitamin A deficiency influences iron homeostasis in cells through affecting the expression of IRP2 and the activity of IRP-RNA comhinatiion and then changing the expressions of ferritin and transferrin mRNA.

关 键 词：维生素A缺乏 铁代谢 铁调节蛋白 铁蛋白 转铁蛋白受体 

分 类 号：R151.44[医药卫生—营养与食品卫生学]