VEGF siRNA致中国仓鼠肺细胞染色体畸变的研究  

Research of chromosome aberration in Chinese hamster lung fibroblast induced by VEGF siRNA

作　　者：荆春霞[1] 张洹[2] 杨光[3] 吴赤蓬[4] 何林[4] 

机构地区：[1]暨南大学医学院流行病学教研室,广州510632 [2]暨南大学医学院血液病学研究所,广州510632 [3]暨南大学医学院寄生虫系,广州510632 [4]暨南大学医学院卫生学教研室,广州510632

出　　处：《卫生研究》2011年第5期576-578,共3页Journal of Hygiene Research

基　　金：广东省科技重点基金资助项目(No.2001-Z-037-01);广东省自然科学基金资助项目(No.021195)

摘　　要：目的研究VEGF siRNA对中国仓鼠肺细胞(CHL)的染色体畸变作用。方法采用25、50和100nmol/L的VEGF siRNA转染中国仓鼠的肺细胞,分别观察24h、48h后的染色体畸变情况。结果在VEGF siRNA转染24小时,仅100nmol/L VEGF siRNA产生可疑阳性反应,其染色体的畸变率为6%;在VEGF siRNA转染48小时后,50nmol/L的VEGF siRNA和100nmol/L的VEGF siRNA均产生可疑阳性反应,染色体的畸变率分别为6%和10%。而25nmol/L的VEGF siRNA无论是在转染后24h还是48h,均未产生染色体的畸变作用。VEGF siRNA产生的染色体畸变类型有断裂、双着丝粒、多倍体、裂隙、三射体。结论 100nmol/L的VEGF siRNA分子可引起CHL细胞产生染色体畸变。Objective To explore the effects of VEGF siRNA on chromosome aberration in Chinese hamster lung fibroblasts（CHL）.Methods The chromosome aberrations were observed after CHL were transfected with VEGF siRNA for 24 hours and 48h.Results There were a questionable positive in 100nmol/L VEGF siRNA after VEGF siRNA were transfected for 24h and the chromosome aberration rate was 6 percentages.Both 50nmol/L VEGF siRNA and 100nmol/L VEGF siRNA caused the questionable positive after VEGF siRNAs were transfected for 48h,and the chromosome aberration rates was 6 percentages and 10 percentages separately.There were no the chromosome aberration in 25nmol/L VEGF siRNA.The types of chromosomal aberration induced by VEGF siRNAs included break,Dicentric grain,polyploid,gap,and three trajectories.Conclusion 100nmol/L VEGF siRNA might cause CHL chromosome aberration.

关 键 词：血管内皮生长因子 VEGF SIRNA 中国仓鼠肺细胞(CHL) 染色体畸变 

分 类 号：R994.6[医药卫生—毒理学] R734.2[医药卫生—药学]