乳清蛋白通过抗氧化作用改善模型大鼠的胰岛素抵抗  

Whey protein improves insulin resistance via the increase of antioxidant capacity in model rats

作　　者：童星[1] 董加毅[1] 邬志薇[1] 李伟[1] 秦立强[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院营养与食品卫生学教研室,苏州215123

出　　处：《卫生研究》2011年第5期617-619,共3页Journal of Hygiene Research

基　　金：国家自然科学基金项目(No.30771808);苏州大学医学部研究生创新基金项目

摘　　要：目的探讨乳清蛋白对胰岛素抵抗的改善作用。方法用高脂饲料制备胰岛素抵抗模型大鼠,将模型大鼠随机分为0%乳清蛋白(WP)组、5%WP组和15%WP组,每组10只。8周后测定大鼠空腹血糖(FBG)、空腹血浆胰岛素水平(FINS),进行葡萄糖耐量试验(OGTT)并计算葡萄糖曲线下面积(AUC),部分血浆用来测定总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)和丙二醛(MDA)等抗氧化指标。结果摄取乳清蛋白没有改变大鼠FBG和葡萄糖AUC。15%WP组大鼠血浆FINS水平和胰岛素抵抗指数(HOMA-IR)显著低于0%WP组(P<0.05)。15%WP组大鼠血浆T-AOC、SOD和GSH显著高于0%WP组(T-AOC:P<0.01;SOD、GSH:P<0.05),同时血浆MDA含量显著降低(P<0.05)。结论乳清蛋白有效改善了模型大鼠的胰岛素抵抗,这可能和增强机体抗氧化能力有关。Objective To investigate the effects of whey protein on insulin resistance in model rats.Methods Insulin resistance model rats were established by feeding high-fat diet in Wistar rats.Model rats were then randomly divided into 4 groups of 10 animals each and fed on 0% whey protein（WP）,5% WP and 15% WP.After 8 weeks,fasting blood glucose（FBG）,fasting plasma insulin（FINS） were determined.Oral glucose tolerance test（OGTT） was performed and glucose area under the curve（AUC） was calculated.Also,plasma total anti-oxidation capacity（T-AOC）,superoxide dismutase（SOD）,reduced glutathione hormone（GSH） and malondialdehyde（MDA） were determined.Results Whey protein intake did not affected FBG and glucose AUC.FINS and homeostatic model assessment-insulin resistance（HOMA-IR） were significantly lower in 15% WP group than in 0% WP group.For antioxidant index,plasma level of T-AOC,SOD and GSH were significantly higher in 15% WP group than in 0% WP group（T-AOC：P0.01;SOD,GSH：P0.05）;however,MDA was significantly lower in 15% WP group than in 0% WP group（P0.05）.Conclusion Whey protein improves insulin resistance.This effect is related to the increase of antioxidant capacity in insulin resistance model rats.

关 键 词：乳清蛋白 胰岛素抵抗 血糖 抗氧化 

分 类 号：R581[医药卫生—内分泌] R151.3[医药卫生—内科学]