一种辅助改善记忆保健食品功能评价的动物模型  

An animal model for evaluating the effect of memory improvement assisted by functional foods

作　　者：卜兰兰[1,2] 石哲[2] 孙秀萍[2,3] 刘新民[2] 赵明耀[1] 高江晖[2,4] 

机构地区：[1]郑州大学基础医学院病理生理教研室,河南郑州450001 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]山东中医药大学,山东济南250355 [4]暨南大学,广东广州510632

出　　处：《中国食品卫生杂志》2011年第5期402-406,共5页Chinese Journal of Food Hygiene

基　　金：国家科技重大专项新药创制(2009ZX09502-014;2009ZX09103-336);国家自然科学基金(30973888)

摘　　要：目的为辅助改善记忆类保健食品的功能评价提供新的动物模型。方法使用滚筒睡眠干扰仪对小鼠进行不同时间(51、0、15 d)睡眠干扰后,用自主活动仪检测自主活动,水迷宫、避暗试验检测认知功能。结果干扰5 d组小鼠的自主活动、水迷宫定位航行和空间探索、避暗训练和检测各指标与对照组比较,差异无统计学意义;干扰10 d组与对照组比较,自主活动总路程、平均速度、运动总时间增加,水迷宫定位航行潜伏期从第4 d起增加,空间探索穿台次数的差异无统计学意义,避暗训练错误次数、潜伏期、暗室路程增加,避暗检测的差异无统计学意义;干扰15 d组与对照组比较,自主活动差异无统计学意义,水迷宫定位航行潜伏期增加、空间探索穿台次数减少,避暗训练错误次数、潜伏期、暗室时间、暗室路程增加,明室时间显著减少,避暗检测差异无统计学意义。结论采用本法,干扰5 d对小鼠自主活动和认知功能无影响,干扰10 d影响小鼠自主活动、损害其空间学习和避暗学习能力,干扰15 d不影响小鼠自主活动、损害其空间学习记忆和避暗学习能力,可为辅助改善记忆类保健食品的功能评价提供新的动物模型。Objective To provide an animal model for the evaluation of memory improvement assisted by functional foods.Methods The behavior performance of mice after sleep interruption（SI） by a rotating drum for 5,10 and 15 days were inspected by Open Field test（OF）,Morris Water Maze test（MWM） and Dark Avoidance test（DA）.Results The activities and cognitive ability of mice were not different between 5d SI group and 5d control group.Comparing the 10d SI group with 10d control group,the total distance,movement speed,total duration of movement in OF were increased,and the latency of place navigation in MWM was increased from Day 4,but there was no difference on the number of crossing in target quadrant.Error times,latency and distance in dark box were increased,but there was no difference in testing course.There was no difference on the activities of 15d SI group and 15d control group in OF,while for the 15d SI group,the latency from Day4 in MWM was increased,and the number of crossing in target quadrant was decreased in spatial probe course.Error times,latency,time and distance in dark box were increased,and the time in light box was significantly decreased in training course of DA,but there was no difference in testing course.Conclusion There was no effect of SI on spontaneous activities and cognition in mice after 5 d SI;Spontaneous activity was affected,and both spatial learning ability in MWM and learning ability in DA were impaired after 10 d SI;Spontaneous activity was not affected,while both spatial learning memory in MWM and learning ability in DA were impaired after 15 d SI.The method could be used for establishing animal models in the evaluation of memory improvement effects of functional foods.

关 键 词：辅助改善记忆 保健食品 自主活动 水迷宫 避暗 ICR小鼠 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]