固相萃取-气相色谱法检测食品中农药残留方法的研究  

Research on detection of pesticide residues in food by solid phase extraction-gas chromatography method

作　　者：陆英洲 陆德胜[2] 

机构地区：[1]杭州娃哈哈集团公司质监部,杭州310014 [2]浙江省疾病预防控制中心,杭州310009

出　　处：《中国卫生检验杂志》2011年第9期2185-2186,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:为寻求最佳食品中农药残留检测方法。方法:采用固相萃取作为农药残留检测前处理,然后采用GC法测定国家食品污染物监测要求的24种农药,包括有机磷13种:甲拌磷、马拉硫磷、对硫磷、甲基对硫磷、毒死蜱、甲基毒死蜱、甲胺磷、久效磷、敌敌畏、乙酰甲胺磷、乐果、氧化乐果、乙拌磷;氨基甲酸酯3种:呋喃丹、甲萘威(西维因)、抗蚜威;菊酯5种:氰戊菊酯、氯氰菊酯、氯菊酯、溴氰菊酯、氟氯氰菊酯;有机氯3种:六六六、滴滴涕、百菊清。结果:将19种农药混合标准溶液在0.005 mg/L～0.05 mg/L0、.05 mg/L～0.5 mg/L和0.1 mg/L～1.0 mg/L三个水平添加到蔬菜和水果样品中进行方法的精密度试验,方法的添加回收率在70%～104%之间,变异系数小于15%。方法的检出限在0.0001 mg/kg～0.005 mg/kg。结论:固相萃取-气相色谱法检测食品中农药多残留方法精密度、回收率满意、确实可行。Objective： To seek the best examination method for the detection of pesticide residues in food.Methods： Solid phase extraction was used for pretreatment of pesticide residue detection and GC was adopted to determine the 24 kinds of pesticide residues,which were requested by national food contamination monitoring.Results： The mixed standard solution composed by 19 kinds of pesticides was added into samples of vegetables and fruits for accuracy test with 3 levels of 0.005 mg/L^0.05 mg/L,0.05 mg/L^0.5 mg/L and 0.1 mg/L^1.0 mg/L.The spiked recovery of the method was between 70%~104% with the coefficient of variation under 15%.The detection limit was in the range of 0.0001 mg/kg^0.005 mg/kg.Conclusion： Solid phase extraction-gas chromatography is available for the detection of pesticide residues in food with its satisfied accuracy and recovery.

关 键 词：固相萃取 气相色谱 农药残留 

分 类 号：O657.71[理学—分析化学]