山药中多酚氧化酶活性的测定及其护色研究  

Study on the Activity of Polyphenoloxidase and Prevention of Discoloration of Dioscorea opposita

作　　者：杜德红[1] 陈勇[1] 肖军霞[1] 

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《安徽农业科学》2011年第27期16572-16574,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究山药中多酚氧化酶活性的测定及其护色效果。[方法]以新鲜山药为试验材料,采用分光光度法测定其多酚氧化酶的活力。同时,通过试验分析不同抑制剂对山药多酚氧化酶活力的影响。[结果]所选护色剂均有不同程度的护色作用,其中以草酸的效果为最佳。[结论]山药加工的最佳复合护色配方为:抗坏血酸10 mg/ml,柠檬酸8 mg/ml,草酸6 mg/ml和亚硫酸氢钠1 mg/ml。[Objective] The aim was to study the activity of polyphenoloxidase and prevention of discoloration of Dioscorea opposite.[Method]Take the fresh Dioscorea opposite as the material,the activity of Polyphenoloxidase was determined by spectrophotometric method.Meanwhile,the effects on the activity of Polyphenoloxidase for the different inhibitors were tested by experiments.[Result] The results showed that the selected color fixatives had the color prevention effect in varying degrees and the best inhibitor was oxalic acid.[Conclusion]The optimal composite color prevention formula of Dioscorea opposita was ascorbic acid 10 mg/ml,sodium bisulfite 8 mg/ml,oxalic acid 6 mg/ml and citric acid 1.00%.

关 键 词：山药 护色 多酚氧化酶 

分 类 号：S567[农业科学—中草药栽培]