大量分离松材线虫幼虫及获得无菌幼虫的方法  

A method of massively isolating PWN larvae and obtaining sterile larvae

作　　者：叶兼菱[1] 张建平[1,2] 陶坚[1] 赵博光[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]江苏科技大学生物与环境工程学院,江苏镇江212018

出　　处：《中国森林病虫》2011年第5期33-35,共3页Forest Pest and Disease

基　　金：根据松萎蔫病致病新学说创立防治新方法及培育抗病新树种(林业公益性行业科研专项No.200704030)

摘　　要：利用松材线虫成虫和幼虫在比重和运动性方面的差异,通过在0.3%羧甲基纤维素钠盐溶液中沉降而得到分离。并在4℃条件下用3%H2O2处理松材线虫幼虫60 min而得到无菌幼虫。There were differences in specific gravity and movement between larva and adult of pine wood nematode in carboxymethyl cellulose sodium salt（CMC） 0.3% solution.And based on that a new method was developed to isolate a large amount of pine wood nematode larvae.And pine wood nematode larvae could be sterilized when treated in hydrogen peroxide 3% at 4 ℃ for 60 min.

关 键 词：松材线虫 酸式滴定管 无菌 幼虫 羧甲基纤维素钠盐 

分 类 号：S763[农业科学—森林保护学]