栀子根化学成分的分离与鉴定  

Isolation and identification of chemical constituents of roots from Gardenia jasminoides Ellis

作　　者：曹百一[1] 刘润祥[1] 王晶[1] 贾凌云[1] 郑乾文 路金才[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]厦门中医院药剂科,福建厦门361009

出　　处：《沈阳药科大学学报》2011年第10期784-787,817,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的对栀子(Gardenia jasminoides Ellis)的根进行化学成分研究。方法应用硅胶柱色谱、ODS柱色谱和Sephadex LH-20柱色谱等分离手段进行分离纯化,根据理化性质及波谱数据鉴定化合物的结构。结果从栀子根体积分数为70%的乙醇提取物中分离得到10个化合物,分别鉴定为桦木酸(betulinic acid,1)、齐墩果酸(oleanolic acid2,)、齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷-6'-O-甲酯(oleanolic acid 3-O-β-D-glucuronopyranoside-6'-O-methyl ester,3)、常春藤皂苷元-3-O-β-D-吡喃葡萄糖醛酸苷-6'-O-甲酯(hederagenin 3-O-β-D-glucuronopyranoside-6'-O-methyl ester,4)、竹节参苷(chikusetsusaponinⅣa,5)、豆甾醇(stigmasterol,6)、β-谷甾醇(β-sitosterol,7)、胡萝卜苷(dau-costerol,8)、香草酸(vanillic acid,9)、丁香酸(syringic acid,10)。结论化合物3～5、8～10为首次从栀子属植物中分离得到,化合物12、7、为首次从栀子根中分离得到。Objective To study the chemical constituents from the roots of Gardenia jasminoides Ellis. Methods The chemical constituents were isolated by various isolation methods （silica gel, ODS and Sepha- dex LH-20 column chromatography） and their structures were elucidated by the analysis of spectral data and chemical properties, Results Ten compounds were isolated from 70% ethanol-water extract and their structures were identified as： betulinic acid（ 1 ）, oleanolic acid（2）, oleanolic acid -3-O-β-D-glucuronopyranoside-6′-O-methyl ester （ 3 ）, hederagenin -3-O-β-D-glucuronopyranoside-6′-O-methyl ester （ 4 ）, chikusetsusaponin IVa（5 ）, stigmasterol（ 6 ） ,β-sitosterol （ 7 ）, daucosterol （ 8 ）, vanillic acid （ 9 ） and syringic acid （ 10 ）. Conclusions Compounds 3- 5,8-10 are isolated from Gardenia genus for the first time, and compounds 1, 2 and 7 are isolated from roots of G. jasminoides Ellis for the first time.

关 键 词：栀子 化学成分 结构鉴定 

分 类 号：R914[医药卫生—药物化学] R284[医药卫生—药学]