含离子液体体系中固定化细胞转化甘草酸生成单葡萄糖醛酸基甘草次酸  

Biosynthesis of glycyrrhetic acid 3-O-mono-β-D-glucuronide by immobilized cells with ionic liquid

作　　者：陈金燕[1] 刘桂艳[2] 黄申[2] 童延斌[1] 李春[1,2] 

机构地区：[1]石河子大学化学化工学院,石河子832003 [2]北京理工大学生命学院,北京100081

出　　处：《生物加工过程》2011年第5期17-21,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20976014);教育部博士点基金资助项目(20091101110036);北京市自然科学基金资助项目(2112035)

摘　　要：研究疏水性离子液体1-丁基-3-甲基咪唑六氟磷酸盐([BMIM]PF6)与醋酸-醋酸钠缓冲液两相体系中,固定化产紫青霉Penicillium purpurogenum Li-3细胞转化甘草酸(GL)生成单葡萄糖醛酸基甘草次酸(GAMG)的反应,并与缓冲液单相体系作为对照。确定了在[BMIM]PF6/缓冲液两相体系中,最适离子液体加入比例、缓冲液pH、反应温度、底物浓度分别为10%、5.83、5℃和6.0 mmol/L,在此条件下反应58 h,甘草酸转化率为87.03%,比缓冲液单相体系提高了15.02%。离子液体循环使用8次后,回收利用率维持在85.28%。主产物GAMG和副产物甘草次酸(GA)在两相体系中得到有效分离,为后续产物分离带来便利。The hydrolysis of glycyrrhizin （GL） to glycyrrhetic acid 3-O-mono-β-D-glucuronide （GAMG） catalyzed by immobilized cell in system containing 1-butyl-3-methylimidazolium hexafluorophosphate （ [ BMIM 3 PF6 ） was performed. The reactions in [ BMIM ] PF6/buffer hiphasic system were studied, compared with the monophasie buffer system. The optimal conditions of reaction in [ BMIM] PF6/buffer biphasic system were determined, the optimum ionic liquid concentration, buffer pH, substrate concentration and temperature were 10% , 5.8, 35 ℃ , and 6.0 mmol/L, respectively. Under the optimized conditions, 87.03% conversion rate of GL was achieved after 58 h in [ BMIM] PF6/buffer biphasic system, compared with the monophasic buffer system, it increased by 15.02%. The ionic liquid [ Bmim] PF6 also remained at higher recovery rate of 85.28% during repeated use for 8 reaction cycles. The [ BMIM] PF6 could improve catalytic efficiency of whole-cell biocatalysis and bring convenience for separation of products.

关 键 词：葡萄糖醛酸苷酶 甘草酸 甘草次酸 离子液体 固定化细胞 

分 类 号：Q814.2[生物学—生物工程]