L-谷氨酸补料分批发酵的研究  

Study on fed-batch fermentation conditons of L-glutamic acid

作　　者：卢宗梅 康传利[2] 张伟国[2] 

机构地区：[1]中粮生物化学(安徽)股份有限公司,蚌埠233010 [2]江南大学工业生物技术教育部重点实验室,无锡214122

出　　处：《生物加工过程》2011年第5期27-31,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA020301)

摘　　要：对谷氨酸棒杆菌(Corynebacterium glutamicum)HCJ46产L-谷氨酸的补料分批发酵条件进行研究。结果表明:最适初糖质量浓度和最佳残糖维持质量浓度分别为100和(10～20)g/L;对发酵控温方式进行研究,确定了最佳温度控制策略为0～8 h维持32℃8、～16 h维持34℃1、6～32 h维持36℃,同时发现相对溶氧控制在30%左右时产酸最高。在以上的优化条件下,L-谷氨酸产量从72 g/L提高到95 g/L,提高了31.9%。Fed-batch fermentation conditions of Corynebacterium ghttamicum HCJ46 for L-glutamic acid production were studied, It was found that the optimal initial glucose concentration and maintained residual sugar concentration were 100 g/L, and 10 -20 g/L, respectively. The fermentation temperature control mode was further investigated. Results showed that the optimal control strategy was controlled as 0 - 8 h at 32 ℃, 8 - 16 h at 34℃, and 16 - 32 h at 36 ℃. It was also found that the highest glutamic acid productivity was obtained when the dissolved oxygen was controlled at 30%. As a result, under the optimization of fermentation conditions, L-glutamic acid productivity increased from 72 g/L to 95 g/L,improved by 29%.

关 键 词：溶氧 分批补料 L-谷氨酸 温度 

分 类 号：TQ922[轻工技术与工程—发酵工程]