基于光纤Bragg光栅的微生物生长过程检测研究  

Detection of Microorganisms Growth Process Based on Fiber Bragg Grating

作　　者：黄德翼[1] 赵明富[1] 罗彬彬[1] 矫雷子[1] 周斌[1] 

机构地区：[1]重庆理工大学电子信息与自动化学院,重庆400054

出　　处：《压电与声光》2011年第5期725-728,共4页Piezoelectrics & Acoustooptics

基　　金：国家自然科学基金资助项目(50876120);重庆市教委;科委基金资助项目(KJ090620;CSTC;2009DA0001-B11)

摘　　要：理论分析光纤Bragg光栅(FBG)生物传感器的折射率与温度传感原理。设计并制作一个单端腐蚀的FBG传感器同时测量折射率与温度,利用数值计算消除温度变化对生物传感器的影响。实验结果表明,消除温度影响的传感Bragg波长的变化量随酵母菌培养时间的增大而增大,在0～10h酵母菌处于生长繁殖的缓慢期,其酵母菌数量少,浓度低,传感器Bragg波长的变化量较小;10～25h酵母菌处于生长繁殖的对数期,此期间酵母菌数量多,浓度高,Bragg波长的变化量快速增大。测量过程中,Bragg波长随时间的变化量与酵母菌生长繁殖过程的增长规律基本一致,实验验证了该传感器用于监测酵母菌生长过程的可行性。The refractive index and temperature sensing principle of the fiber Bragg grating （FBG） biosensor was analyzed theoretically. A single-end etched FBG was designed and fabricated for simultaneous measurement refractive index and temperature. Temperature compensation scheme was proposed by using numerical calculation eliminate temperature changes to the influence of biosensor. Experiment results showed that the variation of Bragg wavelength increased as the yeast training time increased, in 0-10 h the growth of yeast is slow with a little number of yeast and low concentration in this period, the variation of Bragg wavelength slowly increased：In 10-25 h the variation of Bragg wavelength quickly increased, corresponding to the logarithmic growth phase of yeast, during which the concentration of yeast increased. The variation of Bragg wavelength as time increased consistent with the growth and reproduction of yeast as training time increased. Experimental results verified the feasibility of the sensor for monitoring yeast growth process.

关 键 词：光纤Bragg光栅(FBG) 生物传感器 微生物 折射率 酵母菌 

分 类 号：TN253[电子电信—物理电子学]