便携式三聚氰胺快速检测仪的研制与应用  

Development of Portable Raman Spectrometer and Its Application for Determination of Melamine

作　　者：刘峰[1] 邹明强[1] 张孝芳[1] 齐小花[1] 范晓丽[1,2] 董英[2] 朱超[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]江苏大学生物与环境工程学院,镇江212013

出　　处：《分析化学》2011年第10期1531-1536,共6页Chinese Journal of Analytical Chemistry

基　　金：科技部科技支撑计划(No.2009BAK58B01);国际科技合作与交流专项(No.2008DFA40270)资助项目

摘　　要：自主研发了便携式三聚氰胺速检仪,利用纳米增敏表面增强拉曼光谱法,定量检出限为0.5mg/kg;采用相对测量方式可有效克服测量不稳定性。用于原奶、消毒奶、酸奶、奶粉中三聚氰胺检测,线性关系良好。样品前处理步骤简单。通常情况,平均单样检测时间小于2min,仪器检测时间小于30s,尤其适用于现场快速分析。除了可应用于乳及乳制品中三聚氰胺含量速测外,还成功用于橄榄油中掺假成分快速鉴别、养殖水中孔雀石绿快速检测。A portable Raman spectrometer for melamine analysis was developed.The limit of quantitation was raised to 0.5 mg/kg by using surface-enhanced Raman scattering（SERS） technique.Using the relative measurement method,the instability of measurement was effectively improved.Experiments with raw milk,pasteurized milk,yoghourt,milk powder demonstrated that melamine concentration gradient showed good linearity with the spectrometer measured signal.The rapid inspection spectrometer had a good user interface and functions of analysis such as data collection,storage,classification,evaluation of the confidence level,RSD analysis.It was especially possible to have a rapid on-site testing with it＇s small size,portable,simple sample preparation requirements,the average detection time of single sample 2 min and the response time of instrument 30 s.The detection sensitivity,linear relationship and stability of the spectrometer were also good even though under extreme environment such as Tibet Lhasa and Naqu area that is an altiplano higher than 4500 m.The portable Raman spectrometer was also successfully used for the rapid authentication of olive oil and rapid detection of malachite green in fish farming water and other fields.

关 键 词：三聚氰胺 纳米增敏 表面增强 拉曼光谱法 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]