雷公藤内酯醇对β-淀粉样蛋白诱导的大鼠小胶质细胞IL-1β和PGE_2表达的影响  

Influence of triptolide on the expression of interleukin-1β and prostaglandin E_2 in rat microglia induced by beta-amyloid protein

作　　者：李耀斌[1] 聂菁[1] 吕诚[1] 胡小令[1] 薛国勇[1] 鲁纯纠[1] 

机构地区：[1]南昌大学医学院解剖学教研室,江西南昌330006

出　　处：《中国老年学杂志》2011年第18期3521-3523,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助课题(30660073)

摘　　要：目的探讨雷公藤内酯醇对β-淀粉样蛋白(Aβ)1-40诱导的大鼠小胶质细胞白细胞介素-1β(IL-1β)及前列腺素E2(PGE2)表达的影响。方法本实验用Aβ1-40(20μg/ml)诱导体外培养的原代小胶质细胞,建立阿尔茨海默病(AD)细胞模型,并给予不同剂量雷公藤内酯醇(终浓度分别为5、25μg/ml)在不同时程(2 h、12 h、24 h)进行干预,提取细胞培养上清,采用放射免疫学方法检测IL-1β和PGE2的含量。结果加药后12h,模型组IL-1β和PGE2的含量比对照组明显升高(P<0.01);5μg/ml组和25μg/ml组IL-1β的含量比模型组明显减少(P<0.01),5μg/ml组PGE2的含量比模型组明显减少(P<0.01)。结论雷公藤内酯醇对Aβ1-40诱导的小胶质细胞IL-1β和PGE2的表达上调有抑制作用。Objective To explore the influence of triptolide on the expression of interleukin-1β（IL-1β） and prostaglandin E 2（PGE 2） in rat microglia induced by beta-amyloid protein（Aβ）.Methods Microglia was induced by Aβ1-40（20 μg/ml） to establish the AD cellar model,then treated with triptolide（final concentration respectively was 5,25 μg/ml） for 2,12,24 h.The supernatants were collected for detecting the contents of IL-1β and PGE 2 by radioimmunoassay.Results After 12 h of cultivation,the contents of IL-1β and PGE 2 in model group were obviously increased compared with those of control group（P 0.01）.The contents of IL-1β in 5 and 25 μg/ml groups were obviously decreased compared with those of model group（P 0.01）.The content of PGE 2 in 5 μg/ml group was obviously decreased compared with that of model group（P 0.01）.Conclusions Triptolide can suppress the up-expression of IL-1β and PGE 2 in rat microglia induced by Aβ1-40.

关 键 词：雷公藤内酯醇 β-淀粉样蛋白 小胶质细胞 白细胞介素1Β 前列腺素E2 

分 类 号：R749.16[医药卫生—神经病学与精神病学]