脑出血偏瘫大鼠瘫肢正中神经不同时期的超微结构变化  

Observation on the ultra-microstructure of paralysis limb median nerves in rats with cerebral hemorrhage

作　　者：李晓民[1] 赵德明[1] 汪克明[2] 吴生兵[2] 

机构地区：[1]淮北市人民医院神经内科,安徽省淮北市235000 [2]安徽中医学院针灸经络研究所,安徽省合肥230038

出　　处：《国际神经病学神经外科学杂志》2011年第4期306-308,共3页Journal of International Neurology and Neurosurgery

摘　　要：目的观察脑出血大鼠瘫肢正中神经超微结构的变化。方法 Wistar大鼠60只,模型组及假手术各30只,通过胶原酶加肝素联合注射法建立大鼠脑出血动物模型。造模成功后第3天、14天分别分离瘫肢和健肢正中神经,活检、固定,切片、染色,观察正中神经镜下变化(光镜、电镜)。结果无论是模型组还是假手术祖,光镜下计数大鼠正中神经有髓神经髓鞘无明显变化。同时电镜下观察正中神经超微结构未发现有髓神经轴突肿胀、未出现髓鞘脱失、间质水肿等超微结构改变。结论脑出血偏瘫大鼠瘫肢周围神经结构无明显损害。Objective To study the changes of ultra-microstructures of paralysis limb median nerves in rat model of cerebral hemorrhage.Methods Sixty Wistar rats were divided randomly into two groups： model and sham-operated.The cerebral hemorrhage model was prepated by an injection of collagenase plus heparin.Sampling the median nerves from both the paralysis limb and unaffected limb 3 and 14 days after cerebral hemorrhage.The nerve tissues were biopsied,fixed,sectioned and stained.The ultra-microstructures of the median nerves were observed under light and electron microscopes.Results The myelins of median nerves were not obviously changed in both groups under a light microscope.No changes of the ultra-microstructure of median nerves were found under an electron microscope in both groups.Conclusions The ultra-microstructures of paralysis limb median nerves were not obviously impaired in rats with cerebral hemorrhage.

关 键 词：脑出血 正中神经 电镜 大鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学]