SELDI-TOF-MS联合蛋白质芯片技术在病理性近视早期诊断中的应用  

Application of Surface-Enhanced Laser Desorption Ionization Time of Flight Mass Spectrometry and Protein Chip Technology for the Early Diagnosis of Pathologic Myopia

作　　者：陈胜[1] 胡艳红[1] 祁明信[1] 丁媛[1] 

机构地区：[1]福建中医药大学附属第二人民医院,福建福州350003

出　　处：《武汉大学学报（医学版）》2011年第5期634-637,I0003,共5页Medical Journal of Wuhan University

基　　金：福建省教育厅资助项目(编号:JB10083)

摘　　要：目的:运用蛋白质芯片联合表面增强激光解吸附离子化飞行时间质谱(SELDI-TOF-MS)技术检测病理性近视患者血清蛋白质的差异改变,寻找病理性近视特异的生物标志,以期用于病理性近视的早期诊断。方法:使用CM10芯片联合SELDI-TOF-MS技术检测9例病理性近视患者和9例健康对照人血清中蛋白质谱的改变。结果:病理性近视患者与健康人比较,发现有22个蛋白质峰表达有统计学差异(P<0.01),其中10个蛋白峰高表达,12个蛋白峰低表达,对22个差异蛋白峰进一步用遗传算法结合支持向量机模型(SVM)的方法筛选最佳模型,其中M/Z为8 568.752 1,6 114.741 6,2 666.169 1,3 163.504 9,5 990.294 1,6 098.263 0,3 492.5 993,5 953.829 1,6 843.396 7,6 637.371 2,2 873.621 6的蛋白峰为区分两组的SVM模型的标志物。结论:病理性近视患者血清中差异表达的蛋白质较多,SELDI-TOF-MS联合蛋白质芯片技术有望成为病理性近视早期筛查和早期诊断的新的有效工具。Objective：To detect different protein expression in serum between patients with pathologic myopia and healthy people using the SELDI-TOF-MS proteinchip platform,in order to find the specific biomarkers which could be used for the early diagnosis of pathologic myopia.Methods：Serum samples of nine patients with pathologic myopia and nine healthy subjects were tested by CM10 chip on SELDI-TOF-MS proteinchip reader.Results：Twenty-two different protein spots were obtained in serum of pathologic myopia.The expressions were up-regulated in 10 of the 22 protein spots,and were down-regulated in the other 12 spots.The protein spots at m/z of 8 568.752 1,6 114.741 6,2 666.169 1,3 163.504 9,5 990.294 1,6 098.263 0,3 492.599 3,5 953.829 1,6 843.396 7,6 637.371 2,2 873.621 6 are the candidates as pathologic myopia biomarkers in the serum of pathologic myopia patients.Conclusion：There are a few candidates as pathologic myopia biomarkers in the serum of pathologic myopia patients.SELDI-TOF-MS protein chip technology could be a potential method in the clinical screening test of pathologic myopia.

关 键 词：病理性近视 蛋白质芯片 SELDI-TOF-MS 

分 类 号：R778.11[医药卫生—眼科]