CDX-2和LI-cadherin蛋白在胃肿瘤及良性病变中的表达与意义  

Expressions of CDX-2 and LI-cadherin in Gastric Benign Lesions and Tumor and Its Significance

作　　者：刘仕琪[1] 牛建华[2] 吕毅[1] 木拉提[2] 王毅训[3] 辛国宏[4] 

机构地区：[1]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061 [2]新疆石河子大学医学院第一附属医院胃肠外科,新疆石河子832002 [3]新疆石河子大学医学院第一附属医院病理科,新疆石河子832002 [4]西安交通大学医学院第一附属医院肿瘤科,陕西西安710061

出　　处：《中国普外基础与临床杂志》2011年第9期936-941,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金重点项目(项目编号:30830099)~~

摘　　要：目的通过研究胃肿瘤及良性病变中尾相关同源异型盒转录因子-2(caudal type homeobox tran-scription factor-2,CDX-2)与肝肠钙黏蛋白(LI-cadherin)的表达,探讨两者在胃癌发生、发展过程中的可能作用。方法采用免疫组织化学方法分别检测28例正常胃黏膜、30例慢性浅表性胃炎、42例慢性萎缩性胃炎、58例肠化生胃黏膜、46例胃腺癌、30例癌旁组织以及10例胃间质瘤组织中CDX-2和LI-cadherin蛋白的表达,并结合临床病理特征进行分析。结果①CDX-2与LI-cadherin蛋白在正常胃黏膜、慢性浅表性胃炎、癌旁组织以及胃间质瘤组织中均不表达;在肠化生组织中表达阳性率分别为91.4%(53/58)和82.8%(48/58);在胃腺癌组织中表达阳性率分别为80.4%(37/46)和65.2%(30/46),其中肠型胃腺癌中CDX-2和LI-cadherin蛋白表达阳性率(90.6%,29/32;78.1%,25/32)明显高于弥漫型胃腺癌(57.1%,8/14;35.7%,5/14),P<0.05。②CDX-2蛋白表达与Lauren分型(P=0.015)和肿瘤分化程度(P=0.007)有关,而LI-cadherin蛋白表达与Lauren分型(P=0.008)、淋巴结转移(P=0.007)及临床分期(P=0.013)有关。③在肠化生胃黏膜中,细胞核中CDX-2蛋白与细胞膜或细胞浆中LI-cadherin蛋白共同表达阳性率为83.0%(44/53),在胃癌组织中CDX-2与LI-cadherin蛋白共同表达阳性率为67.6%(25/37)。尽管两者表现出很强的伴随关系,但相关性分析却表明两者并无相关性(r=238,P=0.115)。结论 CDX-2和LI-cadherin蛋白的异常表达可能分别与肠化生及胃腺癌的发生和发展有关,两者是否具有协同作用还需进一步研究。Objective To investigate the potential role of caudal type homeobox transcription factor-2（CDX-2） and liver-intestine cadherin（LI-cadherin） in the development and progression of gastric tumor by detecting their expressions in gastric cancer and benign lesions.Methods The expressions of CDX-2 and LI-cadherin protein were detected by immunohistochemistry in normal gastric mucosa（n=28）,chronic superficial gastritis（n=30）,chronic atrophic gastritis（n=42）,intestinal metaplasia（n=58）,gastric adenocarcinoma（n=46）,vicinity cancerous tissue（n=30）,and gastric stromal tumor（n=10）.Then,the relationship between expression of CDX-2 or LI-cadherin protein and clinicopathologic features was analyzed.Results ①The expressions of CDX-2 and LI-cadherin were all negative in normal gastric mucosa,chronic superficial gastritis,vicinity cancerous tissue,and gastric stromal tumor.The positive rates of CDX-2 protein expression in intestinal metaplasia and gastric adenocarcinoma were 91.4%（53/58） and 80.4%（37/46）,respectively,and the positive rates of LI-cadherin protein expression were 82.8%（48/58） in intestinal metaplasia and 65.2%（30/46） in gastric adenocarcinoma.The positive rates of CDX-2 and LI-cadherin protein expression in intestinal type gastric adenocarcinoma（90.6%（29/32） and 78.1%（25/32）） were higher than those in diffuse type gastric adenocarcinoma（57.1%（8/14） and 35.7%（5/14））,P0.05.② CDX-2 protein expression was associated with degree of differentiation（P=0.007）,and LI-cadherin protein expression was associated with lymph node metastasis（P=0.007） and cancer staging（P=0.013）.③ In the mucosa of intestinal metaplasia,the co-expression positive rate of CDX-2 protein expression in nucleus with LI-cadherin protein in cytoplasm or membrane was 83.0%（44/53）,while the co-expression positive rate was 67.6%（25/37） in gastric adenocarcinoma.Although strong tendency between CDX-2 and LI-cadherin protein expression was showed,it

关 键 词：胃肿瘤 胃良性病变 免疫组织化学 尾相关同源异型盒转录因子-2 肝肠钙黏蛋白 

分 类 号：R735.2[医药卫生—肿瘤]