层层自组装构筑｛Hb-ZnO/IP_6｝_6修饰电极及其直接电化学行为  

Direct electrochemistry of {Hb-ZnO/IP_6}_6 modified electrode through layer-by-layer self assembly technology

作　　者：徐欢[1] 王耀[1] 杨海峰[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2011年第4期388-393,共6页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学面上基金项目(20975068)

摘　　要：将纳米氧化锌与血红蛋白(Hb)的混合液,通过肌醇六磷酸酯(IP6)层层自组装于玻碳电极(GCE)表面,制备了{Hb-ZnO/IP6}6修饰电极.用循环伏安法、电化学阻抗光谱和场发射扫描电镜对{Hb-ZnO/IP6}6膜进行了表征.实验结果表明:{Hb-ZnO/IP6}6/GCE呈现层状三维多孔结构,实现了血红蛋白和电极表面的直接电子转移.该修饰电极对过氧化氢有很好的电催化活性,线性范围为2.00×10-6～1.12×10-4mol.L-1,最低检测限为1.40×10-6mol.L-1(信噪比S/N=3),米氏常数为5.1 mmol.L-1.Inositol hexakisphosphate（IP6） and the mixed solution of zinc oxide nanoparticles and hemoglobin（Hb） were alternately modified onto the surface of a glassy carbon electrode（GCE）through layer-by-layer self-assembly technique for constructing {Hb-ZnO/IP6}6/GCE.{Hb-ZnO/IP6}6/GCE was characterized with cyclic voltammetry（CV）,electrochemical impedance spectroscopy（EIS） and field emission scanning electron microscopy（FESEM）.Experimental results showed that the {Hb-ZnO/IP6}6/GCE exhibited three-dimensional porous structure and realized the direct electron transfer between Hb and electrode surface.{Hb-ZnO/IP6}6/GCE displayed excellent electrocatalytic activity for the reduction of H2O2 with a linear response range from 2.00×10-6-1.12×10-4 mol·L-1 and a detection limit of 1.40×10-6mol·L-1（at the ration of signal to noise,S/N=3）.The Michaelis-Menten constant K Mapp was estimated to be 5.1 mmol·L-1.

关 键 词：肌醇六磷酸酯 纳米氧化锌 血红蛋白 层层自组装 直接电子转移 

分 类 号：O647.3[理学—物理化学]