旋毛虫28S rRNA基因片段的克隆及其在分类学上的应用  

Cloning and application of 28S rRNA gene fragment of Trichinella spiralis on Taxonmy

作　　者：李成[1] 魏颖[1] 袁金钱[1] 宋铭忻[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2011年第9期80-83,共4页Journal of Northeast Agricultural University

基　　金："十一五"国家科技支撑计划重大项目(2010BAD04B01);兰州家畜疫病病原生物学国家重点实验室开放课题(SKLVEB2009KFKT005);黑龙江省普通高等学校青年学术骨干支持计划项目(1153G007)

摘　　要：为了探讨所采集旋毛虫的分类,利用PCR方法克隆了猪旋毛虫黑龙江隔离种核糖体28S rRNA序列的基因片段。序列分析结果表明,猪旋毛虫黑龙江隔离种与旋毛形线虫(Trichinella spiralis,T1)的进化关系较近,确定为旋毛形线虫(Trichinella spiralis)。结果与传统的分类结果基本一致,为传统的分类学方法提供了新的理论依据。In order to investigate the classification of Trihicnella swine isolate from Heilongjiang Province,the gene fragment in ribosome 28S rRNA was cloned and sequenced.The results showed that Trihicnella swine isolate from Heilongjiang Province was closed and belonged to Trichinella spiralis by sequence analysis.To some extent,the result was consistent with the traditional classfication and provided a base for the traditional taxonomy.

关 键 词：旋毛虫 28S RRNA 隔离种 

分 类 号：S852.71[农业科学—基础兽医学]