测定猪肉中土霉素及金霉素残留的HPLC法建立  

Determination of residues of oxytetracycline and chlortetracycline in pork by HPLC

作　　者：智若岚[1] 刘中成 李广兴[1] 张瑞莉[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]哈尔滨市兽药饲料监察所,哈尔滨150040

出　　处：《东北农业大学学报》2011年第9期141-144,共4页Journal of Northeast Agricultural University

基　　金：黑龙江省青年基金项目(QC08C96);黑龙江省教育厅科研项目(10531005)

摘　　要：文章建立了测定猪肉组织中土霉素及金霉素残留量的HPLC法。该方法按照国家检测标准采用了5%高氯酸50 mL为样品提取液;乙腈＋0.01 mol·L-1磷酸二氢钠溶液（用30%硝酸调节pH2.5）=35 65为流动相;检测波长为355 nm,进行测定。在此色谱条件下,土霉素、金霉素标准品浓度在0.1~1.0μg·mL-1、0.2~2.0μg·mL-1范围内线性关系良好,在3个不同浓度添加水平的回收率为78.06%~87.21%,变异系数为3.02%～7.72%,金霉素的回收率为76.45%~85.62%,变异系数为3.46%~6.32%。土霉素及金霉素的最低检测线为20和10μg·kg-1。该检测方法简便、快速且灵敏度高,适用于土霉素、金霉素残留的定性定量分析。To establish an HPLC method for determination of oxytetracycline and chlorotetracycline in pork.According to the national detective standard,5% perchloric acid（50 mL） was adopted as sample extract,acetonitrile＋0.01 mol.L-1 sodium dihydrogen phosphate as mobile phase and 355 nm as detection wavelength.In this condition,the result showed that the standard substances concentration of oxytetracycline and chlorotetracycline linear correlation good to extent 0.1-1.0 μg.mL-1 and 0.2-2.0 μg.mL-1 on this chromatographic column condition,oxytetracycline： different concentration average recoveries were feom 78.06% to 87.21%,and RSD was 3.02%-7.72%.Chloroteracycline： different concentra-tion average recoveries was 76.45%-85.62% and RSD was 3.46%-6.32%.The lowest detectable limits of oxytetracycline and chlorotetracycline were 20 and10 μg.kg-1.The results indicated that the method presented was of convenience,simpleness,quickness and high sensitivity.And the method was applicable to proof quantitative analysis of oxytetracycline and chlorotetracycline residue.

关 键 词：高效液相色谱法 土霉素 金霉素 猪肉 残留检测 

分 类 号：S828[农业科学—畜牧学]