扫描电镜的不同含水量植物叶片样品的处理及观察方法研究  

Study on sample treatment methods of plant leaves with different water contents for SEM

作　　者：侯燕鸣[1] 胡剑江[1] 方蔷[2] 张倩[3] 雷洪涛[1] 王毅[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]中国医科大学附属第四医院呼吸科,沈阳110032 [3]吉林大学药学院,长春130021

出　　处：《分析仪器》2011年第5期45-48,共4页Analytical Instrumentation

基　　金：国家自然科学基金资助项目(No30600842);财政部自主选题研究项目(Nozx-zzxt-2006-005;2010ZZ005)

摘　　要：为探讨不同含水量植物叶片进行扫描电镜观察的最合适的样品处理方法,及不同电子束加速电压对扫描电镜图像清晰度的影响,对不同含水量叶片进行了比较性研究。并通过理论分析得出:含水量小于75%的植物叶片可不经处理,直接进行观察,大于79%的叶片必须经过样品制备后观察;5-15KV加速电压时获得的图像最清晰。In order to select the appropriate sample treatment metholds of plant leaves for SEM observation, and to investigate the effect of electron beam accelerating voltage on SEM image resolution, plant leaves with different water contents were observed with SEM and the results were compared. It was showed that the leaves with water contents less than 75% could be observed directly, while leaves with water contents greater than 79% should be observed after treatment. The images obtained at accelerating voltages of 5-15kV had the highest resolution.

关 键 词：扫描电镜 样品处理 叶片含水量 图像清晰度 加速电压 

分 类 号：Q94-33[生物学—植物学]