单宁微球固载α-淀粉酶及其催化性能  

Immobilized α-Amylase on Tannin Microspheres and Its Catalytic Performance

作　　者：卢玉栋[1] 林晨霞[1] 叶琳[1] 吴宗华[1] 

机构地区：[1]福建师范大学化学与材料学院,福建福州350007

出　　处：《高分子材料科学与工程》2011年第9期157-160,共4页Polymer Materials Science & Engineering

基　　金：福建师范大学横向项目(DH-584)

摘　　要：以单宁微球为载体,采用戊二醛交联法固定α-淀粉酶。探讨了最佳固定化条件,并考察了温度、pH值和淀粉初始浓度等对固定α-淀粉酶催化淀粉水解的影响。结果表明,单宁微球对α-淀粉酶的吸附率达到了96.99%。最佳固定条件为:戊二醛质量分数0.10%,pH5.5,温度25℃,时间2h。固定α-淀粉酶的重复5次后仍具有较好的催化效果。α-amylase was immobilized on tannin microspheres by linking with glutaraldehyde.The optimum conditions for immobilization were studied.The effects of temperature,pH and initial starch concentration on the catalytic performance of immobilized enzyme were studied.The results show that the adsorption of α-amylase by tannin microspheres reaches 96.99%.The optimum conditions for immobilization are： the mass fraction of glutaraldehyde is 0.10%,pH is 5.5,temperature is 25 ℃,time is 2 h.The immobilization enzyme still had a good catalytic performance on hydrolysis of starch after having been reused repeatedly 5 times.

关 键 词：单宁微球 Α-淀粉酶 固定化 催化水解 

分 类 号：TQ426.97[化学工程]