外源性NO在细胞水平对GluR6 S-亚硝基化的作用  

Effect of exogenous NO on the S - nitrosylation of GIuR6 in vitro

作　　者：底洁卉[1,2] 张光毅[2] 

机构地区：[1]徐州医学院肿瘤生物治疗实验室,江苏徐州221002 [2]徐州医学院生物化学与分子生物学研究中心

出　　处：《徐州医学院学报》2011年第9期577-579,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金（30870543）

摘　　要：目的观察外源性一氧化氮（NO）供体硝普钠（SNP）和亚硝基谷胱甘肽（GSNO）在细胞水平对GluR6 S-亚硝基化的影响。方法将本事构建的pEGFP—GluR6质粒转染HEK293细胞24h后，加不恫浓度外源性NO供体SNP和GSNO处理30min后收集蛋白，用生物素转换法检测GluR6S—亚硝基化水平。结果给了不同浓度SNP时GluR6的s一亚硝基化均增高，并存给予1mmol／LSNP时其亚硝基化水平达最高。另外，给予200μmoL／L GSNO时GluR6的S-亚硝基化也增高。结论在过表达GIuR6的HEK293细胞中，外源性NO供体SNP和GSNO均能使GluR6 S-亚硝基化水平增高。结果提示，在体外，外源性NO能直接诱导GluR6 S-亚硝基化。Objective To observe the effect of exogenous nitric oxide （NO） donors sodium nitropreside （SNP） and S - nitrosoglutathione （GSNO） on the S - nitrosylation of GluR6 in vitro. Methods Biotin - Switch method was applied to observe the effect on S - nitrosylation of GIuR6 through treatment with various concentration of SNP or GSNO for 30 min at 37℃ in HEK293 cell transiently expressing the pEGFP - GluR6 receptor. Results The S - nitrosylation level of GluR6 all increased with different concentrations of SNP, and reached a highest level at 1 mmol/L treatment. The level also increased with 200 μmol/L GSNO treatment. Conclusion Both of exogenous NO donors SNP and GSNO can increase the S - nitrosylation of GluR6 in HEK293 cell transiently expressing the pEGFP - GluR6 receptor, indicating that exogenous NO can directly increase the S - nitrosylation of GluR6 in vitro.

关 键 词：GLUR6 硝普钠 亚硝基谷胱甘肽 一氧化氮 HEK293细胞 

分 类 号：R364.12[医药卫生—病理学]