高产大花生及其高油突变体DGAT1基因的克隆与序列分析  

Cloning and Sequencing of DGAT1 Gene from High Yielding Large-seeded Peanut Genotypes and Their High Oil Peanut Mutants

作　　者：房超琦[1] 杨传得[1] 于洪涛[2] 唐月异[2] 王秀贞[2] 吴琪[2] 王传堂[2] 王丕武[1] 

机构地区：[1]吉林农业大学,吉林长春130118 [2]山东省花生研究所,山东青岛266100

出　　处：《花生学报》2011年第3期7-10,15,共5页Journal of Peanut Science

基　　金：国家花生产业技术体系(CARS-14);青岛市科技发展计划项目(09-1-3-67-jch;10-3-3-20-nsh);山东省自然科学基金(Y2008D11)

摘　　要：以高产大花生花育22号和LF2及其高油突变体为材料利用PCR扩增DGAT1基因,并对其序列进行了比较、分析,鉴定出长、短不同的序列,并进行了系统发育分析。为进一步研究高油特性与DGAT1之间的关系奠定了基础。DGAT1 gene was cloned from high-yielding peanut genotypes Huayu 22, LF2 and their high oil mutants using PCR. Sequences were compared, long sequences and short sequences were identified, and a phylogenetic tree was constructed. The study paved the way to understanding the relationship between high oil trait and DGAT1. K

关 键 词：花生 高油突变体 DGAT1基因 基因克隆 序列分析 

分 类 号：S565.2[农业科学—作物学] Q782[生物学—分子生物学]