广西百色地区壮族男性人群骨密度与脂联素基因单核苷酸多态性的关系  

Association between adiponectin gene single nucleotide polymorphisms and bone mineral density in a male population of Zhuang nationality in Guangxi Baise area

作　　者：王金花[1] 黄秀峰[1] 周庆辉[1] 林朝文[1] 杨园园[1] 韦叶生[2] 黄昌盛[1] 吴联滔[3] 吴玉梅[3] 何兰凤[1] 

机构地区：[1]右江民族医学院,人类学研究所,广西壮族自治区百色市533000 [2]右江民族医学院附属医院,广西壮族自治区百色市533000 [3]右江民族医学院,广西壮族自治区百色市533000

出　　处：《中国组织工程研究与临床康复》2011年第33期6252-6256,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(No.30860117);广西科技计划项目(桂科攻0898004)桂科计字[2008]98号;右江民族医学院攻关项目[2008]2号~~

摘　　要：背景:脂联素可在骨代谢中发挥重要作用。目的:观察脂联素基因单核苷酸多态性与广西百色地区壮族男性骨密度的关系。方法:选取广西百色地区壮族男性跟骨骨量减少患者,采用单碱基延伸的单核苷酸多态性分型技术对广西百色地区302例壮族男性的脂联素基因的5个单核苷酸多态性位点(rs1063539、rs12495941、rs266729和rs3774261)进行了基因分型。结果与结论:以5个多态性位点作为自变量的多元Logistic回归检测结果显示,仅rs3774261多态性与跟骨超声振幅衰减显著相关(OR=1.948,95%CI:1.184～3.203,P<0.01),并独立于骨量减少的传统危险因素。对基因型进行纯合子与杂合子合并后的协方差分析显示,仅rs3774261的AG+GG与AA基因型的跟骨超声振幅衰减值差异有显著性意义(P<0.05),AG+GG型对骨密度具有一定的保护作用,AA型是骨密度降低的危险因素。结果证实,脂联素基因第2内含子rs3774261位点多态性与中国百色地区壮族男性骨密度有一定关联。BACKGROUND：Studies have shown that adiponectin plays an important role in bone metabolism,but the relationship between adiponectin gene polymorphism and bone metabolism in osteoporosis has rarely been reported.OBJECTIVE：To investigate the correlation between adiponectin gene 5 single nucleotide polymorphisms and bone mineral density in Guangxi Zhuang nationality men.METHODS：A case-control study was carried out on osteopenia patients.Genotypes for adiponectin gene 5 loci（rs1063539,rs12495941,rs266729,rs3774261 and rs710445）polymorphism in 302 Guangxi Zhuang nationality men were determined by Multiplex SNaPshot.RESULTS AND CONCLUSION：rs1063539,rs12495941,rs266729 and rs710445 polymorphisms were met with Hardy-Weinberg equilibrium（P 0.05）.Five loci genotype frequencies in the normal group and the osteopenia group met with Hardy-Weinberg equilibrium（P 0.05）.There was no significant difference in the genotype distributions of five loci polymorphism between low bone mass group and control group（P 0.05）.After adjustments for age,weight,height,movement and body mass index,multivariate Logistic regression analyses revealed that only rs3774261 polymorphism remained significantly associated with low bone mass（P 0.01）.The subjects with the combined AA genotype had higher risk of low bone mass compared with those with the AG＋GG genotype（adjusted OR =1.948,95%CI：1.184-3.203,P=0.009）.Polymorphism of rs3774261 was independently correlated with calcaneal low bone mass in Baise Zhuang nationality male population.The results confirm that,adiponectin gene intron 2 rs3774261 polymorphism and bone mineral density in Baise Zhuang nationality men have some relevance.The presence of the AA genotype may dominantly increase the risk of osteopenia.AG＋GG genotype has a protective effect on bone mineral density.The data also suggest that the rs1063539,rs12495941,rs266729 and rs710445 polymorphism might not be associated with osteopenia in Baise Zhuang nationality men.

关 键 词：单核苷酸多态性 脂联素 骨质疏松症 遗传 骨密度 多重单碱基延伸 多态性 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]