缺血性脑卒中患者血清GFAP、NDKA和PARK7的临床应用价值  

Clinical application of serum GFAP,NDKA and PARK7 in patients with ischemic stroke

作　　者：崔桂萍[1] 刘萍[2] 杨萍[1] 陆卉[2] 梁建伟[1] 张葳[1] 

机构地区：[1]天津市环湖医院检验科,300060 [2]天津市环湖医院神经内科,300060

出　　处：《中华检验医学杂志》2011年第9期779-784,共6页Chinese Journal of Laboratory Medicine

摘　　要：目的探讨血清GFAP、NDKA和PARK7与IS的相关性，及其对IS诊断、预后评估的临床价值。方法用ELISA法检测37例IS患者、28例ICH患者和30名健康人血清GFAP、NDKA和PARK7含量水平，所有患者在发病12h内、第3、14天除进行上述3项指标检测外，还在相应时间点用MESSS进行神经功能缺损评分，在14d出院时用Barthel指数（BI）评价其日常生活能力。同时分析3项指标单项检测和联合检测对IS的诊断效能。结果Is组发病12h内、第3、14天血清GFAP含量分别为（5．49±2．25）、（5．17±2．29）、（5．96±2．39）μg/L，血清NDKA含量分别为9．15（6．28～12．79）、9．13（6．31-12．23）、9．31（6．40～11．83）μg／L，血清PARK7含量分别为（32．71±6．34）、（31．23±6．04）、（32．79±6．94）μg／L；健康对照组血清GFAP、NDKA、PARK7含量分别为（4．62±1．56）、4．24（3．30～5．61）、（14．25±2．65）μg／L。IS组与健康对照组比较，除第3天的GFAP差异无统计学意义（t=1．129，P〉0．05），IS组其余各时间点的3项指标水平分别高于健康对照组（GFAP的t值分别为2．642、1．870，P均〈0．05；NDKA的Z值分别为6．173、6．100、6．278，P均〈0．01；PARK7的t值分别为14．964、15．367、16．060，P均〈0．01）。检测GFAP对IS诊断的特异度和敏感度分别为46．7％（14／30）和81．1％（30／37），NDKA对IS诊断的特异度和敏感度分别为90．0％（27／30）和78．4％（29／37），PARK7对IS诊断的特异度和敏感度分别为96．7％（29／30）和97．3％（36／37）；3项标志物联合检测对Is诊断的特异度和敏感度分别为96．7％（29／30）和100％（37／37）。此外，IS组GFAP升高的发病风险是健康对照组的1．3倍（OR=1．300，P=0．044），NDKA升高是1．7倍（OR=1．668，P=0．036），PARK7则是1．8倍（OR=1．809，P=0．005）。IS患者发病〈12h时GFAP血清含量在Is组和ICH组Objective To explore the relationship of GFAP,NDKA and PARK7 serum concentrations of patients with IS, and their diagnose and prognosis value in IS. Methods The serum concentrations of GFAP, NDKA and PARK7 were detected in 37 IS patients, 28 ICH patients and and 30 healthy persons by ELISA. These indexes of patients were detected in 12 hours, 3 d and 14th day after onset of ischemic stroke. Their neurological injury status were also evaluated by MESSS at corresponding time points, and their activities of daily living were evaluated by BI at 14 d discharge from hospital. At the same time, the diagnostic efficiency was analysed for IS using the three biomarkers and the combined detection. Results In IS group, the serum concentrations of GFAP in 12 hours, 3rd and 14th day after onset were （5.49 ±2. 25） μg/L, （5.17±2. 29） Ixg/L and （5.96± 2. 39） μg/L, respectively. The serum concentrations of NDKA were 9. 15 （6. 28 - 12.79） μg/L, 9. 13 （ 6. 31 - 12. 23 ） μg/L, 9. 31 （ 6. 40 - 11.83 ） μg/L respectively, and the serum concentrations of PARK7 were （ 32. 71± 6. 34 ） μg/L, （ 31.23 ± 6. 04） μg/L, （ 32. 79 ± 6. 94）μg/L respectively. The serum levels of GFAP, NDKA and PARK were respectively （4. 62 ± 1.56） μg/L, 4. 24（3.30-5.61）μg/L, （14. 25 ±2. 65） μg/L in healthy control group. The levels in IS groups were remarkably increased compared with the healthy control group except the level of GFAP in the 3rd day （t = 1. 129, P 〉 0.05 ）. The levels in other time points were significantly different between patients group and healthy control, t value of GFAP were respectively 2. 642, 1. 870, P 〈 0. 05 ; Z value of NDKA were 6. 173, 6.100, 6. 278,P 〈0. 01; t value of PARK7 were 14. 964, 15.367,16.060,P 〈0. 01. The specificity and sensitivity of the individual detection for diagnosis of IS was 46.7% （14/30） and 81.1% （30/37） for GFAP, 90.0% （27/30）and 78.4% （29/37） for NDKA, 96. 7% （29/30）and 97.3% （36/37） for PARK7. The specificity a

关 键 词：卒中 脑缺血 神经胶质原纤维酸性蛋白质 NM23核苷二磷酸激酶类 细胞内信号肽和蛋白质类 

分 类 号：R743.3[医药卫生—神经病学与精神病学]