白细胞介素-10对小鼠弓形虫眼病眼部炎症的影响  

Effect of Interleukin-10 in Inflammation of Murine Ocular Toxoplasmosis

作　　者：蔄雪静[1,2] 吴欣怡[1] 李元彬[2] 于华军[2] 孙英[2] 张凤兰[2] 

机构地区：[1]山东大学齐鲁医院眼科,山东济南250012 [2]烟台毓璜顶医院眼科,山东烟台264000

出　　处：《中山大学学报（医学科学版）》2011年第5期664-668,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：烟台市科技发展计划(2011年)

摘　　要：【目的】探讨白细胞介素-10(IL-10)对弓形虫眼病小鼠视网膜组织炎症和体内γ-干扰素(IFN-γ)和肿瘤坏死因子-α(TNF-α)的影响。【方法】将120只C57BL/6小鼠分为3组:①对照组40只;②感染组(实验A组)40只;③IL-10干预组(实验B组)40只。腹腔注射弓形虫感染小鼠后,观察小鼠眼部的组织学切片,对眼球和外周血做基因检测;注射IL-10后,观察其对眼部炎症反应和体内IFN-γ和TNF-α水平的影响。【结果】弓形虫感染第6天,在实验组小鼠眼部和外周血中均可检测到小鼠P30基因;房水中IFN-γ(4531.4 pg/mL)和TNF-α(1572.3 pg/mL)的浓度升高;血清中IFN-γ(456±39)pg/mL和TNF-α(14.6±2.2)pg/mL的浓度升高;视网膜组织可见到弓形虫,且出现明显的炎症反应;IL-10注射后,房水中IFN-γ(2482.7 pg/mL)和TNF-α(1248.1 pg/mL)的浓度降低,血清中IFN-γ(266±27)pg/mL和TNF-α(12.1±1.9)pg/mL的浓度降低,但仍高于对照组,视网膜组织中仍可见到弓形虫,但炎症反应减轻,将三组血清中两种细胞因子的浓度组间差异进行方差分析,P<0.01,表明差别具有统计学意义。【结论】小鼠急性眼部弓形虫感染病变主要集中在视网膜,IL-10可减轻弓形虫感染小鼠的眼部炎症反应。【Objective】 To understand the effect of interleukin-10 in retinal inflammation,the concentration of IFN-γ（interferon-γ） and TNF-α（tumor necrosis factor-α） in vivo of murine ocular toxoplasmosis.【Methods】 One hundred and twenty C57BL/6 mice were divided into three groups： forty mice in the control group,forty mice in the infection group（experiment A） and forty mice in the injecting IL-10 group（experiment B）.Toxoplasma gondii infected mice by intraperitoneal injection.Histological sections of the three groups were detected.The DNA of Toxoplasma gondii in eyes and serum were detected.The concentration of IFN-γ and TNF-α in eyes and serum were detected in experimental groups.【Results】 P30 gene could be detected in experiment groups on the sixth day.Compared with the control group,the concentration of IFN-γ（4531.4 pg/mL） and TNF-α（1572.3 pg/mL） in aqueous humor was higher in experiment A group;and the concentration of IFN-γ（456 ± 39 pg/mL） and TNF-α（14.6 ± 2.2 pg/mL） in serum was higher in experiment A group too.Toxoplasm and apparent inflammation were watched in retina.Compared with the experiment A group,the concentration of IFN-γ（2482.7 pg/mL） and TNF-α（1248.1 pg/mL） in aqueous humor was lower in experiment B group,and the concentration of IFN-γ（266 ± 27 pg/mL） and TNF-α（12.1 ± 1.9 pg/mL） in serum was lower in experiment B group too.Mean square analysis about the concentration of IFN-γ and TNF-α in serum was carried out between the three groups.The difference was statistical significance（P 0.01）.Toxoplasm and light inflammation were watched in retina.【Conclusion】Toxoplasma gondii damaged retina.IL-10 could relieve inflammation of murine ocular toxoplasmosis.

关 键 词：弓形虫眼病 白细胞介素-10 炎症 Γ-干扰素 肿瘤坏死因子-Α 

分 类 号：R774[医药卫生—眼科]