肝细胞癌中Survivin与NF-кB蛋白的表达及其临床意义  

Protein expression and significance of survivin and NF-κB in hepatocellular carcinoma

作　　者：金亦[1] 邵春奎[1] 唐录英[1] 杨建勇[2] 黄勇慧[2] 李家平[2] 

机构地区：[1]中山大学附属第三医院病理科,广州510630 [2]中山大学附属第一医院介入放射科

出　　处：《中华医学杂志》2011年第36期2542-2545,共4页National Medical Journal of China

基　　金：广东省科技计划项目（20098030801027）;广东省自然科学基金（7001663）

摘　　要：目的初步探讨Survivin和NF—κB蛋白表达在肝细胞癌发生中的作用及其临床意义。方法对确诊的206例肝细胞癌组织标本及癌旁正常肝组织应用免疫组化法进行Survivin和NF—κB蛋白表达的检测，分析肝细胞癌中两种蛋白表达与病理参数及预后的关系。结果Survivin主要表达在细胞质，NF—κB主要表达在细胞核。206例肝细胞癌组织中Survivin和NF—κB蛋白的阳性表达率分别为78．2％和81．1％，均明显高于正常肝组织（11．7％和15．5％）（均P〈0．01）；肝细胞癌中Survivin蛋白表达与包膜侵犯、门静脉癌栓、淋巴结转移及临床分期有关（P〈0．01）；NF—κB蛋白表达与门静脉癌栓、淋巴结转移及临床分期有关（P〈0．05）。肝细胞癌中Survivin与NF—κB蛋白表达呈正相关（r=0．52，P〈0．01）；肝细胞癌组织中Survivin和NF-κB表达全阳性的患者1、3年生存率分别为70．3％和30．4％，均明显低于两者表达全阴性者（88．5％和61．5％）（均P〈0．05）。结论Survivin和NF—κB蛋白在肝细胞癌中高表达，两者在肝细胞癌的发展中相互促进，可作为预后的重要指标。Objective To detect the protein expressions of survivin and NF （nuclear factor）-κB and investigate their roles in the pathogenesis of hepatoeellular carcinoma. Methods A total of 206 cases were diagnosed as hepatocellular carcinoma, and immunohistoehemical technique was used to detect the protein expressions of survivin and NF-KB in hepatocellular carcinoma samples and normal liver tissue specimens. Statistical analysis was performed to analyze the relationship of protein expressions of survivin and NF-κB and clinico-pathological parameters and prognosis. Results The expression of survivin was predominant in cytoplasm while that of NF-κB was in nucleolus. The positive rate of survivin protein was 78.2% in hepatocellular carcinoma and that of NF-κB was 81.1%. The positive rate of survivin and NF-κB was higher in hepatocellular carcinoma than that in benign hepatic tissue （11.7% and 15.5% ）. Significant differences existed in the expressions of survivin and NF-κB between hepatocellular carcinoma and normal hepatic tissue （ P 〈 0. 01 ）. The expression of survivin was related to amieula invasion, portal vein tumor thrombus, lymph node metastasis and clinical stage in hepatocellular carcinoma （P 〈 0. 01 ）. The expression of NF-κB was also related to portal vein tumor thrombus, lymph node metastasis and clinical stage in hepatocellular carcinoma （ P 〈 0. 05 ）. There was an obviously positive correlation between the expressions of survivin and NF-KB in hepatocellular carcinoma （ r = 0. 52, P 〈 0. 01 ）. Survival rate of patients for 1, 3 year of positive expression of survivin and NF-κB in hepatocellular carcinoma was 70. 3% and 30. 4% , respectively. And it was obviously lower than that of those of negative expression （88. 5% and 61.5% ）. There was significant difference in the expressions of survivin and NF-κB between positive and negative subjects in 1, 3 year （ P 〈 0. 05 ）. Conclusion Protein expressions of survivin and NF-κB are higher in hepatoeellular carcinoma.

关 键 词：癌 肝细胞 NF—κB 存活蛋白 预后 

分 类 号：R735.7[医药卫生—肿瘤]