骨桥蛋白对大肠癌细胞间隙连接通信的影响  

Effects of osteopontin on gap junctional intercellular communication in colon cancer cell lines

作　　者：丁凌[1] 胡涵光[1] 郑树[2] 

机构地区：[1]浙江大学医学院附属第二医院肿瘤科,杭州310009 [2]浙江大学肿瘤研究所

出　　处：《中华医学杂志》2011年第36期2578-2581,共4页National Medical Journal of China

摘　　要：目的检测骨桥蛋白（Osteopontin，OPN）转染后大肠癌SW-480细胞株的细胞间隙连接通信（gap junctional intercellular communication, GJIC）改变。方法以OPN真核表达质粒转染大肠癌SW-480细胞株后，利用激光共聚焦显微镜（laser scanning confocal microscope, LSCMM），运用荧光淬灭后恢复技术（flurescence redistribution after photobleaching, FRAP）检测OPN转染后SW-480细胞的GJIC功能变化。结果转染OPN后，高表达OPN的SW-480-pcDNA3．1（＋）-OPN细胞株淬灭5rain后荧光恢复率为24．65％±4．08％，而对照组SW-480-pcDNA3．1（＋）为44．74％±6．23％，P〈0．001；10min后荧光恢复率SW-480-pcDNA3．1（＋）-OPN仅为25．98％±4．48％，SW-480-pcDNA3．1（＋）已上升至64．92％±5．39％，P〈0．001。转染后高表达OPN的SW-480细胞，GJIC功能被抑制，细胞之间通信减弱，淬灭后恢复率相比较对照组明显降低。结论OPN减弱大肠癌细胞间的通信能力，可能与大肠癌细胞的侵袭转移相关。Objective Liver metastasis is a major cause of mortality in patients with colorectal cancer. However, the mechanisms underlying this process remain largely unknown. Osteopontin （OPN） is a secreted phosphorylated glyeoprotein known to be involved in tumor migration and metastasis. Gap junction intercellular communication （GJIC） is a pathway of direct cell to cell communication. This study analyzed gap junctional intercellular communication （GJIC） changes in colon cancer SW-480 cell lines after osteopontin had been transfeeted in. Methods Transfect sense-osteopontin eukaryotic expression plasmids into SW-480 cell lines and detect the GJIC changes in two different cell lines by flurescence redistribution after photobleaching （FRAP）. Results Compared with control, signal exchange is impaired and GJIC function is inhibited in SW-480-pcDNA3.1 （ ＋ ） -OPN cell lines. FRAP after 5 min in SW480-pcDNA3.1 （ ＋ ）-OPN is 24. 65% ± 4. 08%, the control SW-480-pcDNA3. 1 （ ＋ ） is 44. 74%± 6. 23%, P 〈 0. 001 ; FRAP after 10 min in SW-480-pcDNA3.1 （ ＋ ） -OPN is 25.98% ±4.48% , while SW-480-peDNA3.1 is up to 64. 92% ± 5.39% ,P 〈 0. 001. Conclusion OPN could inhibit GJIC function of colon cells to accelerate the metastatic process in colorectal cancer.

关 键 词：结肠肿瘤 骨桥蛋白 细胞间隙连接通信 

分 类 号：R735.34[医药卫生—肿瘤]