Using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to analyze differentially expressed brain polypeptides in scrapie strain 22L-infected BALB/c mice  

作　　者：Xiangyu Liao Jiayu Wan Wensen Liu Xin Tang Wufei Zhu Na XU Jing Xu Nan Li Yaping Chang Chuanjing Ju 

机构地区：[1]Department of Immunology, Norman Bethune College of Medical Science, Jflin University, Changchun 130021, Jllin Province, China [2]The First Clinical Medical College of Three Gorges University, Yichang Central Hospital, Yichang 443003, Hubei Province, China [3]Institute of Military Veterinary Sciences, Academy of Military Medical Sciences, Changchun 130122, Jilin Province, China [4]College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin Province, China [5]The Fourth Hospital of Jilin University, Changchun 130011, Jilin Province, China

出　　处：《Neural Regeneration Research》2011年第23期1801-1805,共5页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China,No. 30972197 and 31072148;Science and Technology Plan Program of Jilin Province,No. 201105038

摘　　要：Differentialiy expressed polypeptides in the brain of a BALB/c mouse model infected with scrapie strain 22L were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results showed that 21 peptides were down-regulated, with peptides of mass-to-charge ratio 758.772 5 and mass-to-charge ratio 5 432.206 9, demonstrating the most significant decreases. These finding suggest that these peptides are candidate biomarkers and may play an important role in the pathogenesis of prion disease.Differentialiy expressed polypeptides in the brain of a BALB/c mouse model infected with scrapie strain 22L were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results showed that 21 peptides were down-regulated, with peptides of mass-to-charge ratio 758.772 5 and mass-to-charge ratio 5 432.206 9, demonstrating the most significant decreases. These finding suggest that these peptides are candidate biomarkers and may play an important role in the pathogenesis of prion disease.

关 键 词：prion diseases misfolded prion protein imaging mass spectrometry PEPTIDES biological markers mass spectrometry 

分 类 号：O633.14[理学—高分子化学] O657.63[理学—化学]