Ad-Egr-hTrail联合放射线对脑胶质瘤细胞杀伤作用的实验性研究  

Experimental study of Ad-Egr-hTrail combined with radiation on human glioma cell line

作　　者：刘桂红[1,2] 章龙珍[1,2] 唐天友[1,2] 刘美艳[3] 赵丽 

机构地区：[1]徐州医学院附属医院放疗科 [2]徐州医学院肿瘤放射治疗教研室,221006 [3]徐州医学院第三附属医院 [4]连云港第四人民医院

出　　处：《中国实用医药》2011年第26期1-3,共3页China Practical Medicine

基　　金：徐州市科技项目(项目编号:XF10C078);徐州医学院院科研课题(项目编号:08KJ25)

摘　　要：目的探讨重组腺病毒联合X射线对U251脑胶质瘤细胞的杀伤作用。方法实验分6组:空白对照组(A组),Ad-Egr-hTrail组(B组),Ad-CMV-hTrail组(C组),单独照射组(D组),Ad-Egr-hTrail+照射组(E组),Ad-CMV-hTrail+照射组(F组)。取对数生长期的U251细胞接种于96孔细胞培养板中,48h后重组腺病毒感染U25细胞,感染2d后分别给予D、E、F组12GyX射线一次性照射,照射后3d,MTS法测定细胞抑制率。结果①B组与A组相比差异无统计学意义(P>0.05),其余各实验组与对照组相比差异有统计学意义(P<0.01)。②两种腺病毒重组载体单独使用时,C组的抑制率明显高于B组(P<0.01),两者联合放疗时,抑制率均明显提高(P<0.01),E组是B组的7.43倍,F组是C组的1.84倍。③与D组相比,E组、F组的抑制率明显提高(P<0.01),E组的放疗增敏比为1.38倍,F组的放疗增敏比为1.53倍。结论①Ad-Egr-hTrail具有很强的辐射诱导特性。②TRAIL基因具有辐射增敏作用。③两种腺病毒重组载体与放射治疗有正协同增敏作用.Objective To explore the synergistic induction of apoptosis by the combination of Ad-Egr-hTrail and radiation in U251 cell line.Methods The experiment was devided into six groups：control group（A group）, Ad-Egr-hTrail group（B group）, Ad-CMV-hTrail group（C group）,radiation group（D group）, Ad-Egr-hTrail combined with radiation group（E group）, Ad-CMV-hTrail combined with radiation group （F group）.U251 cells were seeded in 96-well plates. Recombinant adenovirus and radiation were used on the U251 cells respectively and jointly.The inhibition rate was measured three days after radiation by MTS assay. Results ①Except B group,every experimental group was significantly different from control group （P0.01）.②When recombinant adenovirus vector was used alone, the inhibition rate of Ad-CMV-hTrail was significantly higher than that of Ad-Egr-hTrail（P0.01）. The inhibition rate of combined therapy was significantly higher than that of the respective application of gene therapy （P0.01）.The inhibition rate of E group was B group 7.14 times and F group was C group 1.84 times.③The inhibition rate of combined therapy group was significantly higher than that of radiation group（D group） （P0.01）. The inhibition rate of E group was D group 1.38 times and F group was D group 1.53 times.Conclusion ①Recombinant adenovirus Ad-Egr-hTrail has the enhanced expression properties induced by radiation.②The Trail gene can increase radiosensitization of tumor cells. ③The anti-tumor effect of gene therapy combined with radiotherapy is better than that of radiotherapy or gene therapy alone.

关 键 词：Ad-Egr-hTrail 基因-放射治疗 胶质瘤 辐射 

分 类 号：R739.41[医药卫生—肿瘤]