原癌基因pokemon的mRNA在肾癌中的表达及其意义  

Expression of Pokemon mRNA and Its Clinical Significance in Renal Cell Carcinoma

作　　者：侯列军[1] 郑军华[2] 耿江[2] 顾闻宇[2] 王波[2] 

机构地区：[1]宁波大学附属医院泌尿外科,浙江宁波315020 [2]同济大学附属上海市第十人民医院泌尿外科,上海200072

出　　处：《中国现代医生》2011年第27期17-19,33,共4页China Modern Doctor

摘　　要：目的检测肾癌组织中pokemon的mRNA的表达,分析其与各临床病理特征间的关系。方法收集47例肾细胞癌手术切除的癌、癌旁及临近正常组织,应用逆转录实时聚合酶链反应(Real-time PCR)检测标本中pokemon基因的表达,并对其与肾癌的临床病理特征进行分析。结果 Real-time PCR检测肾细胞癌、癌旁及正常对照组织中pokemon的mRNA表达有差异,具有统计学意义(P<0.01)。肾细胞癌组织中pokemon的mRNA表达与肿瘤病理分级有关,而与患者的性别、年龄、肿瘤最大直径、TNM临床分期及病理学类型无明显相关性。结论 pokemon的mRNA表达和肾细胞癌的发病及恶性程度有关,可能成为肾癌治疗的有效靶点。Objective To explore the expression of pokemon in human renal cell carcinoma and its correlation with clinical features. Methods Reverse transcription Real-time PCR applied to detect the expression of pokemon in 47 cases of resection samples, their corresponding paracancerous samples and normal samples. Correlation between expression of pokemon and clinical-pathological features was analyzed. Results The expression of pokemon mRNA was significantly higher in the cancerous tissues than those in the paracancerous tissues and normal tissues （P ~ 0.01 ）. The expression of pokemon was related to tumor differentiation degree, but has no correlation with patient＇ s gender, age, maximum diameter of tumor, pathological type and TNM stages. Conclusion Pokemon is expressed in human renal cell carcinoma and it may play a key role in the genesis of renal cell cancer. It may be the therapeutic target of renal cell carcinoma in the future.

关 键 词：POKEMON 肾细胞癌 实时聚合酶链反应 

分 类 号：R737.11[医药卫生—肿瘤]