低振幅振动训练对帕金森小鼠黑质多巴胺能神经元的保护  

The Neuro-protective Effects on the Substantia NigraDopaminergic Neurons in Parkinson's Disease Model of Mice by Low Amplitude Vibration Training

作　　者：何凌骁[1] 赵丽[1] 龚丽景[1] 王德刚[1] 

机构地区：[1]北京体育大学,北京100084

出　　处：《北京体育大学学报》2011年第9期44-47,共4页Journal of Beijing Sport University

基　　金：教育部博士点专项基金"运动改善神经毒的机制探讨"(200800431001);国家体育总局课题"Rho GTPases信号在有氧运动促进神经元突触可视性的作用"(10B029)

摘　　要：目的:观察4周低振幅低频振动训练和低振幅高频振动训练对PD模型小鼠黑质区酪氨酸羟化酶(TH)表达和纹状体区脑源性神经营养因子(BDNF)含量的影响,探讨振动训练改善PD模型运动功能的可能作用机制。方法:32只雄性C57BL/6J小鼠随机分为生理盐水(NS)、PD模型、PD+低振幅低频振动训练组(LFV)、PD+低振幅高频振动训练组(HFV),每组6只。后两组鼠分别接受每天低振幅低频、高频振动训练1次,训练4周。经4周干预后,免疫组织化学检测黑质(SNc)区酪氨酸羟化酶(TH)表达变化,酶联免疫法(ELISA)检测纹状体区BDNF含量。结果:PD组黑质区TH阳性细胞数及纹状体区BDNF含量均较NS组明显减少(P<0.01),而LVT组和HVT组其值均较PD组明显增加(P<0.01),后两组与NS组间以上指标无统计学差异。相关分析显示PD模型组黑质区TH细胞计数与纹状体区BDNF含量呈正相关(r=0.970,P<0.01)。结论:振动训练运动方式使基底神经节环路产生适应性调节,利于PD小鼠模型黑质多巴胺能神经元的存活,而上调纹状体区BDNF可能是其作用机制之一。Objective： to investigate the effects of low amplitude vibration training on potential compensatory changes of tyrosine hydroxylase（TH） in the number of substantia nigra neurons and brain-derived neurotrophic factor（BDNF） in the striatum of MPTP-lesioned mouse,and to discuss the possible molecular mechanisms for the LVT.Methods： Thirty-two male C57BL/ 6J mice were equally randomized into normal saline（NS）,PD model,PD plus low amplitude,low frequency vibration training（LVT） and PD plus low amplitude,high frequency vibration training（HVT）.Vibration training（VT） was started 7 d after MPTP lesioning and continued for 4 W.After the training of VT,the changes in positive TH and the content of BDNF of animals were observed by immunohistochemical technique and ELASA respectively.Results： Compared with NS group,the numbers of TH immunoreactive cells and BDNF values in PD were significantly lower than those in the NS group（all P0.01）;the numbers and the values in LVT group and HVT group were significantly higher compared with those in the PD.（all P0.05）;and the numbers were not significantly different between the two groups and NS group（P0.05）.Correlation analysis showed a positive correlation between the count of TH and BDNF values（r=0.970,P0.01）.Conclusion： It is suggested that VT may lead to improvement in motor performance through adaptive changes of the basal ganglia circuitry,that is beneficial to the survival of dopaminergic neurons in the substantia nigra of PD mice,and the one mechanism might be related to the up-regulation of BDNF expression in striatum.

关 键 词：振动训练 帕金森病 黑质 纹状体 酪氨酸羟化酶 脑源性神经营养因子 

分 类 号：R742.5[医药卫生—神经病学与精神病学]