血管内皮生长因子C在肿瘤相关巨噬细胞中的表达与乳腺癌淋巴管新生及淋巴转移的关系  

The Relationship of Vascular Endothelial Growth Factor-C Expression in Tumor-associated Macrophages with Lymphangiogenesis and Lymphatic Metastasis in Breast Cancer

作　　者：谭海栋[1] 傅晓艳[2] 丁明星[2] 王瑞权[1] 陈志梅[1] 李旭升[2] 

机构地区：[1]金华职业技术学院附属医院,浙江金华321000 [2]金华职业技术学院医学院,浙江金华321007

出　　处：《肿瘤学杂志》2011年第8期599-604,共6页Journal of Chinese Oncology

基　　金：金华市科学技术研究计划项目(2007-3-054)

摘　　要：[目的]探讨肿瘤相关巨噬细胞(TAM)表达的血管内皮生长因子C(VEGF-C)在人乳腺癌淋巴管新生及转移中的作用。[方法]采用免疫组织化学双重染色法检测75例乳腺癌组织TAM计数、TAM中VEGF-C表达率,以及癌内、癌周和正常乳腺组织中D2-40阳性微淋巴管密度(LMVD)和淋巴管内皮细胞增殖(LECP),并计数癌细胞淋巴管侵入(LVI),分析它们之间的相关性。[结果]在乳腺癌TAM中VEGF-C呈强阳性表达,且主要分布于癌周。TAM计数为(133.96±46.96)/(×200,0.74mm2)。VEGF-C在TAM中的阳性表达率为26.56%±13.93%。癌周LMVD和LECP分别为12.99±7.97、6.24%±4.00%,显著高于癌内及正常乳腺组织(P<0.05)。癌周LMVD与TAM计数、VEGF-C阳性表达率呈正相关(r=0.528,P=0.001;r=0.874,P<0.001);癌周LECP与TAM计数、VEGF-C阳性表达率呈正相关(r=0.849,P<0.001;r=0.413,P<0.001)。淋巴结转移阳性组LMVD和LECP分别为15.36±8.36和8.98%±2.92%,显著高于阴性组(P<0.05)。LVI阳性组的LMVD和LECP分别为18.12±9.06和11.11%±6.76%,显著高于阴性组(P<0.001)。[结论]TAM在肿瘤微淋巴管新生中起重要角色,可能通过分泌VEGF-C来诱导癌周组织中LECP,从而LMVD增加,最终增加了乳腺癌淋巴转移的可能性。[Purpose] To investigate the role of vascular endothelial growth factor-C（VEGF-C） expression in tumor-associated macrophages（TAM） in lymphangiogenesis and lymphatic metastasis in breast cancer.[Methods] The specimens of 75 cases of breast carcinoma were monitored by immunohistochemical double-staining technique.The counts of TAM,the VEGF-C expression in TAM,the D2-40 positive lymphatic micro-vessel density（LMVD） and proliferating lymphatic endothelial cells（LECP） in intratumoural and peritumoural areas as well as in normal tissue,and lymph vessel invasion（LVI） were detected.The correlations between them were analyzed.[Results] The VEGF-C was strongly positive expression in TAM of breast cancer,and mainly distributed in peritumoral area.The average counts of TAM was（133.96±46.96）/（×200,0.74mm2）,and the positive rate of VEGF-C expression in TAM was 26.56%±13.93%.LMVD and LECP in peritumoral areas was 12.99±7.97 and 6.24%±4.00% respectively,with significantly higher than those in intratumoural areas and normal tissue（P0.05）.There were positive correlations between the counts of TAM and peritumoral LMVD（r=0.528,P=0.001）,the VEGF-C expression in TAM and peritumoral LMVD（r=0.874,P0.001）,the counts of TAM and peritumoral LECP（r=0.849,P0.001） the VEGF-C expression in TAM and peritumoral LECP（r=0.413,P0.001）.The LMVD and LECP were 15.36±8.36 and 8.98%±2.92% in cases with lymph node metastasis respectively,with significantly higher than that without lymph node metastasis（P0.05）.The LMVD and LECP were 18.12±9.06 and 11.11%±6.76% in cases with LVI,with significantly higher than that without LVI（P0.001）.[Conclusions] TAM play an important role in tumor lymphangiogenesis,it might be via VEGF-C secreting to induce LECP in peritumoral areas,finally result in increasing the risk of lymphatic metastasis in breast cancer.

关 键 词：乳腺肿瘤 肿瘤相关巨噬细胞 血管内皮细胞生长因子C 淋巴管新生 

分 类 号：R737.9[医药卫生—肿瘤]