非洲山毛豆种子中鱼藤酮类化合物的含量测定  

Deternation of Rotenoids Content in T.vogelii Hook f.Seed

作　　者：胡林子[1,2] 李新华[3] 马永全[2] 于新[2] 

机构地区：[1]广东省平远县质量技术监督局,广东平远514600 [2]仲恺农业工程学院轻工食品学院,广州510225 [3]沈阳农业大学食品学院,沈阳110866

出　　处：《中国食品学报》2011年第6期206-211,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：广东省科技计划项目(2008B030302001)

摘　　要：分别采用高效液相法和紫外分光光度法测定山毛豆种子中鱼藤酮及其类似物的含量。两种试验方法均以鱼藤酮为对照品。用紫外分光光度法在其最大吸收波长294nm处进行测定。高效液相色谱法以zorbax eclipse plus C18 (4.6mm×250mm,5μm)为色谱柱,流动相为V(甲醇):V(水)=69:31,柱温25℃,流速0.8mL/min,进样量10μL,检测波长294nm。试验结果表明,紫外分光光度法测定鱼藤酮类物质含量为24.29mg/g,高效液相色谱法测定鱼藤酮含量为1.49mg/g。方法学考察显示,UV法具有方法简便、快速、准确、经济等特点,是一种检测非洲山毛豆种子鱼藤酮类物质含量的较好手段。HPLC法操作相对复杂,但专属性强,重现性好,回收率高,是一种测定鱼藤酮有效含量的方法。Determination of rotenone and its analogues in T.vogelii hook f.seed were made by HPLC method and UV Spectrophotometry.Rotenone was reference substance in both methods,and the maximum absorbance wavelength of UV Spectrophotometry was 294 nm.The conditions of HPLC were chromatographic column of zorbax eclipse Plus C18（4.6 mm×250 mm,5 μm）,column temperature of 25 ℃,mobile phase of MeOH-H2O（69：31,V/V）,flow rate of 0.8 mL/min,injection volume of 10 μL,and UV detection wavelength of 294 nm.The content which obtained by experiment of rotenone and its analogues were 1.49 mg/g and 24.29 mg/g.The methodological evaluation showed that UV spectrophotometry was easy to operate,rapid,reliable and low-cost which can be used for the determination of rotenoids.HPLC method was more complicated,speciality,reproducible and high recovery as an effective method of determining rotenone.

关 键 词：紫外分光光度法 高效液相色谱法 非洲山毛豆种子 鱼藤酮 

分 类 号：S482.3[农业科学—农药学]