乙型肝炎病毒RT区变异及其与基因型分布的关系  

Mutation in the RT Region of Hepatitis B Virus and Its Relationship with HBV Genotypes Distribution

作　　者：周霞[1] 况雪梅[1] 汤影之[1] 邓国宏[1] 王宇明[1] 

机构地区：[1]重庆市第三军医大学西南医院传染科,重庆400038

出　　处：《现代生物医学进展》2011年第19期3605-3609,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金重点项目(2008ZX10002-007)

摘　　要：目的:探讨大样本乙型肝炎病毒(HBV)感染患者RT区耐药位点变异的流行情况,及各耐药位点变异与HBV基因型的关系。方法:采用P区测序法对1117例慢性乙型肝炎患者的血清病毒进行P区测序、进化树分型。结果:RT区耐药位点变异发生率与基因型关系密切,在基因型C患者中的变异发生率远远高于基因型B患者(P=0.000)。Rt180、rtM204V、rtM204I、rt181、rt213位点变异均与基因型C有关(P<0.05)。主要的三种变异类型rt180+rtM204V、rtM204I、rt180+rtM204I间基因型分布存在显著差异(P=0.003)。不同HBeAg状态下,耐药变异的发生有显著差异(P=0.020),特别是rt181和rt236位点变异。结论:HBV基因型影响RT区耐药变异发生率及变异类型,且耐药变异发生率也与HBeAg状态有关。Objective： To investigate the prevalence of resistance mutations in the RT region in a large sample of patients with hepatitis B virus （HBV）, and the relationship between resistance mutation and HBV genotypes. Methods： RT region of I-IBV gene in 1117 patients was sequenced and HBV genotypes were analyzed by phylogenetic tree. Results： The resistance mutation rate in the RT re- gion was closely related to HBV genotypes, and it was obviously higher in genotype C than in genotype B （P=0.000）. The variation of rtl80, rtM204V, rtM204I, rtl81, rt213 position was related with genotype C （P〈0.05）. The distribution ofHBV genotypes in three major types of variation, rtl80＋rtM204V, rtM204I, rtl80＋rtM204I, was obviously different （P=0.003）. HBeAg is relevant to the originate of drug resistance mutation （P = 0.020）, especially rtl81 and rt236 position. Conclusions： HBV genotypes influenced the incidence and types of resistance variation in RT region, and resistance variation incidence was also related to HBeAg state.

关 键 词：乙型肝炎 基因型 突变 RT区 

分 类 号：R512.62[医药卫生—内科学]