Stathmin/Oncoprotein18(Op18)基因在人骨肉瘤中的表达及其意义  

Expression and Significance of Stathmin/Oncoprotein 18(Op18) Gene in Human Osteosarcoma

作　　者：张大鹏[1] 张圣洁[1] 于大淼 富勇[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001 [2]伊春市第一医院,黑龙江伊春153000

出　　处：《现代生物医学进展》2011年第19期3709-3712,共4页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(No.11551168)

摘　　要：目的:探讨stathmin/oncoprotein 18(Op18)基因在人骨肉瘤中的表达规律及意义。方法:分别采用免疫组化法和Western blot法检测10例正常骨组织和56例骨肉瘤中stathmin蛋白的表达。结果:免疫组化法检测stathmin蛋白在正常骨组织、低级别骨肉瘤(G1级组)及高级别骨肉瘤(G2级组)中阳性表达率分别为20%、65%、100%。正常骨组织分别与G1级组、G2级组比较,均有显著性差异(P﹤0.05);G1级组与G2级组比较,有显著差异(P<0.05)。Western blot法检测显示,stathmin蛋白在正常骨组织、G1级组、G2级组表达相对值分别为(0.34±0.15)、(0.68±0.21)、(0.90±0.17)。正常骨组织分别与G1级级组、G2级组比较,差异均有显著性(P<0.01),G1级组与G2级组比较,差异有显著性(P<0.01)。结论:Stathmin在骨肉瘤中过表达,与骨肉瘤的发生及发展密切相关。Objective： To investigate the expression of stathmin/oncoprotein 18 （Op18） in human osteosarcoma and its relation to biological behaviors ofosteosarcoma. Methods： The stathmin protein expressions of 56 cases with human osteosarcoma and 10 cases of normal bone tissues were examined by immunohistochemical staining and Western blot methods. Results： The positive rate of stathmin in normal human bone tissues, G1 grade and G2 grade were 20%, 65%, 100% respectively. The difference between normal human bone tissues and G1 grade, G2 grade were significant （P〈0.05）respectively; the difference between G1 grade and G2 grade were significant（P〈0. 05）. Expression of stathmin in normal human bone tissues, GI grade and G2 grade were （0.34± 0.15）, （0.68± 0.21 ）, （0.90± 0.17） respectively. The difference be-tween normal human bone tissues and G1 grade, G2 grade were significant （P〈0.01） respectively; the difference between G1 grade and G2 grade were significant （P〈0.01）. Conclusion： Overexpression of stathmin plays an important role in development and progression of human osteosarcoma.

关 键 词：骨肉瘤 stathmin/oncoprotein 18 免疫组化法 WESTERN BLOT 

分 类 号：R738.1[医药卫生—肿瘤]